Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7034 | 3' | -52 | NC_001876.1 | + | 15183 | 0.66 | 0.742432 |
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Target: 5'- aGGCGGAgcgcaaaauuAAGcgCGGCuCCagaaAGCGGGCc -3' miRNA: 3'- gCUGCUU----------UUCaaGCCGuGG----UCGUCCG- -5' |
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| 7034 | 3' | -52 | NC_001876.1 | + | 28900 | 0.66 | 0.742432 |
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Target: 5'- gGugGuuGAGcaUUCucaggccuGGC-CCAGCGGGCa -3' miRNA: 3'- gCugCuuUUC--AAG--------CCGuGGUCGUCCG- -5' |
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| 7034 | 3' | -52 | NC_001876.1 | + | 25459 | 0.66 | 0.764234 |
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Target: 5'- aGAgGAGGAGggCGagggcGCGCCGcGCuGGCa -3' miRNA: 3'- gCUgCUUUUCaaGC-----CGUGGU-CGuCCG- -5' |
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| 7034 | 3' | -52 | NC_001876.1 | + | 15430 | 0.66 | 0.774915 |
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Target: 5'- gGACGGAGGGagCGcGCGCC---GGGCg -3' miRNA: 3'- gCUGCUUUUCaaGC-CGUGGucgUCCG- -5' |
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| 7034 | 3' | -52 | NC_001876.1 | + | 26393 | 0.66 | 0.785432 |
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Target: 5'- cCGGCGGucccgaguaGGGGUUgaGGUAgaCGGCGGGCa -3' miRNA: 3'- -GCUGCU---------UUUCAAg-CCGUg-GUCGUCCG- -5' |
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| 7034 | 3' | -52 | NC_001876.1 | + | 8707 | 0.66 | 0.785432 |
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Target: 5'- aGACGAAugacUUCGGCuaCGGCGGcGCg -3' miRNA: 3'- gCUGCUUuuc-AAGCCGugGUCGUC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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