Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7108 | 5' | -61 | NC_001895.1 | + | 18302 | 0.69 | 0.17322 |
Target: 5'- cAGCGCACgGGCGGUggCAUaCGCgGUg -3' miRNA: 3'- -UCGCGUGgCCGCCGuaGUG-GUGgCAg -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 20551 | 0.68 | 0.214805 |
Target: 5'- aGGCGCGCCagcucGGCGaCAaUACCGCCGcCu -3' miRNA: 3'- -UCGCGUGG-----CCGCcGUaGUGGUGGCaG- -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 20640 | 0.78 | 0.035861 |
Target: 5'- uGCGCACCGGCuGCAUCAUCuCCGa- -3' miRNA: 3'- uCGCGUGGCCGcCGUAGUGGuGGCag -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 20704 | 0.69 | 0.181409 |
Target: 5'- cAGCGUgACCGGCguugccgccugaauGGCAUCAacauccCCACCGg- -3' miRNA: 3'- -UCGCG-UGGCCG--------------CCGUAGU------GGUGGCag -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 21457 | 0.69 | 0.187395 |
Target: 5'- cGGUGCACUGGCgcuuacuGGCAUCAUCGgUGcCa -3' miRNA: 3'- -UCGCGUGGCCG-------CCGUAGUGGUgGCaG- -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 21688 | 0.67 | 0.251381 |
Target: 5'- -aCGCACCGGCgacaauGGCAgcCACCACgGcCa -3' miRNA: 3'- ucGCGUGGCCG------CCGUa-GUGGUGgCaG- -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 23052 | 0.74 | 0.078826 |
Target: 5'- gGGCGCACCGGaUGuaGUCACCAuCCG-Cg -3' miRNA: 3'- -UCGCGUGGCC-GCcgUAGUGGU-GGCaG- -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 29959 | 0.67 | 0.244946 |
Target: 5'- aGGUGUgggaGCCGGUaacgaaGUAUCACCACCGg- -3' miRNA: 3'- -UCGCG----UGGCCGc-----CGUAGUGGUGGCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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