Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7108 | 5' | -61 | NC_001895.1 | + | 11579 | 0.69 | 0.187901 |
Target: 5'- aGGUcauUACCGGCGGCAcaggccagcaUCACCGcCCGUg -3' miRNA: 3'- -UCGc--GUGGCCGCCGU----------AGUGGU-GGCAg -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 13403 | 0.68 | 0.220568 |
Target: 5'- cGCGCugUGGUGGCGUcCugUGCgGUa -3' miRNA: 3'- uCGCGugGCCGCCGUA-GugGUGgCAg -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 29959 | 0.67 | 0.244946 |
Target: 5'- aGGUGUgggaGCCGGUaacgaaGUAUCACCACCGg- -3' miRNA: 3'- -UCGCG----UGGCCGc-----CGUAGUGGUGGCag -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 21688 | 0.67 | 0.251381 |
Target: 5'- -aCGCACCGGCgacaauGGCAgcCACCACgGcCa -3' miRNA: 3'- ucGCGUGGCCG------CCGUa-GUGGUGgCaG- -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 4746 | 0.67 | 0.251381 |
Target: 5'- cAGUGCAUCaaGGCuGGCAuaaUCACCGCCc-- -3' miRNA: 3'- -UCGCGUGG--CCG-CCGU---AGUGGUGGcag -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 5254 | 0.66 | 0.285655 |
Target: 5'- gGGCGgcuuccCGCUGcGCGGuCAUCGCUGCCGa- -3' miRNA: 3'- -UCGC------GUGGC-CGCC-GUAGUGGUGGCag -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 1765 | 0.66 | 0.292937 |
Target: 5'- uGGCgacaGCGCCGGauGCGUgguacuggCACCGCCGg- -3' miRNA: 3'- -UCG----CGUGGCCgcCGUA--------GUGGUGGCag -5' |
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7108 | 5' | -61 | NC_001895.1 | + | 5744 | 1.09 | 0.000126 |
Target: 5'- cAGCGCACCGGCGGCAUCACCACCGUCa -3' miRNA: 3'- -UCGCGUGGCCGCCGUAGUGGUGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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