Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7166 | 3' | -61.3 | NC_001900.1 | + | 40660 | 0.66 | 0.332 |
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Target: 5'- uGCCgUGCCacucccaguccgcaCGGUGCAGCUCCGG-GGUg -3' miRNA: 3'- -CGG-ACGG--------------GUCGCGUCGAGGCUgUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 42744 | 0.68 | 0.248824 |
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Target: 5'- gGCCgUGgCCGGUGCGGCUgcgCCGACcaGGUUg -3' miRNA: 3'- -CGG-ACgGGUCGCGUCGA---GGCUG--UCGA- -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 43458 | 1.08 | 0.000221 |
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Target: 5'- uGCCUGCCCAGCGCAGCUCCGACAGCUc -3' miRNA: 3'- -CGGACGGGUCGCGUCGAGGCUGUCGA- -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 46954 | 0.66 | 0.334381 |
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Target: 5'- cGCgCUGaaguaCAGCGaCAGgUUCGACAGCUu -3' miRNA: 3'- -CG-GACgg---GUCGC-GUCgAGGCUGUCGA- -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 47859 | 0.67 | 0.28207 |
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Target: 5'- uGCCUGCguugCCGGUGCcuaaaccgccccGGCcgcuUCCGGCGGCc -3' miRNA: 3'- -CGGACG----GGUCGCG------------UCG----AGGCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 48710 | 0.68 | 0.275149 |
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Target: 5'- aGCCgcGCUUcaGGCGCGGCgcuagaUCCGGCGGUUg -3' miRNA: 3'- -CGGa-CGGG--UCGCGUCG------AGGCUGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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