Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7166 | 3' | -61.3 | NC_001900.1 | + | 33272 | 0.67 | 0.288415 |
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Target: 5'- gGCCUucugGCCCAGCuucagcaGCGGCUucagcUCGACGGUc -3' miRNA: 3'- -CGGA----CGGGUCG-------CGUCGA-----GGCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 22135 | 0.67 | 0.288415 |
|
Target: 5'- aGCUcGCUCAGCGCggugaaacugaGGCUgaaggccagaccgCCGACGGCg -3' miRNA: 3'- -CGGaCGGGUCGCG-----------UCGA-------------GGCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 47859 | 0.67 | 0.28207 |
|
Target: 5'- uGCCUGCguugCCGGUGCcuaaaccgccccGGCcgcuUCCGGCGGCc -3' miRNA: 3'- -CGGACG----GGUCGCG------------UCG----AGGCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 48710 | 0.68 | 0.275149 |
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Target: 5'- aGCCgcGCUUcaGGCGCGGCgcuagaUCCGGCGGUUg -3' miRNA: 3'- -CGGa-CGGG--UCGCGUCG------AGGCUGUCGA- -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 25898 | 0.68 | 0.261717 |
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Target: 5'- aUCUGCUCGGC-CGGCUCaaCGACGGUc -3' miRNA: 3'- cGGACGGGUCGcGUCGAG--GCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 21025 | 0.66 | 0.36732 |
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Target: 5'- gGCCUGUgaCGGUgGCGGCUCCGcaccCGGUUa -3' miRNA: 3'- -CGGACGg-GUCG-CGUCGAGGCu---GUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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