Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7166 | 3' | -61.3 | NC_001900.1 | + | 34670 | 0.67 | 0.311129 |
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Target: 5'- aGUCcGCCCAGUGCAucaucgccauGCgagCCGACcGGCg -3' miRNA: 3'- -CGGaCGGGUCGCGU----------CGa--GGCUG-UCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 6314 | 0.67 | 0.289127 |
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Target: 5'- aCCUGgUCGGCGCAGCcgcaCCGGCcacGGCc -3' miRNA: 3'- cGGACgGGUCGCGUCGa---GGCUG---UCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 32408 | 0.68 | 0.255204 |
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Target: 5'- gGCCUugUCGGCGUcggAGC-CCGACAGCUc -3' miRNA: 3'- -CGGAcgGGUCGCG---UCGaGGCUGUCGA- -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 2567 | 0.69 | 0.207795 |
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Target: 5'- aGCC-GCUgagGGCGUAGC-CCGACGGCg -3' miRNA: 3'- -CGGaCGGg--UCGCGUCGaGGCUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 25472 | 0.76 | 0.073448 |
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Target: 5'- gGCCUGagccgCCAGCGUGGCUCCcACGGCc -3' miRNA: 3'- -CGGACg----GGUCGCGUCGAGGcUGUCGa -5' |
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| 7166 | 3' | -61.3 | NC_001900.1 | + | 17088 | 0.66 | 0.358879 |
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Target: 5'- gGCCUcacCCCGGUcuuccaGCAGCUCgGugGGUUg -3' miRNA: 3'- -CGGAc--GGGUCG------CGUCGAGgCugUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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