Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7183 | 3' | -55.3 | NC_001900.1 | + | 48958 | 0.67 | 0.676879 |
Target: 5'- uCCUCGGU--UGCUGggcaucguguGCC-CCUCGAgGc -3' miRNA: 3'- -GGAGCCAugACGAC----------UGGuGGAGCUgC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 46921 | 0.66 | 0.740928 |
Target: 5'- cCCUCGGg--UGCguuucGACCGcgagaaagcCCUCGAUGg -3' miRNA: 3'- -GGAGCCaugACGa----CUGGU---------GGAGCUGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 43446 | 0.71 | 0.442974 |
Target: 5'- gUCUCGGaucACUGCuUGACCACCcccgcgucauggaUCGGCc -3' miRNA: 3'- -GGAGCCa--UGACG-ACUGGUGG-------------AGCUGc -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 42945 | 0.71 | 0.434292 |
Target: 5'- aCUCGGg---GUUGGCUGCCUCGAUc -3' miRNA: 3'- gGAGCCaugaCGACUGGUGGAGCUGc -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 42732 | 0.66 | 0.740928 |
Target: 5'- uCCUCGaUGucGUUGACCAUCUCG-CGg -3' miRNA: 3'- -GGAGCcAUgaCGACUGGUGGAGCuGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 42294 | 0.66 | 0.73046 |
Target: 5'- gCUCGau-CUGCagGAUCAgCUCGGCGa -3' miRNA: 3'- gGAGCcauGACGa-CUGGUgGAGCUGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 41847 | 0.76 | 0.225097 |
Target: 5'- cCCUCGGUGaucggacGCUGGCCAagCUCGAUGa -3' miRNA: 3'- -GGAGCCAUga-----CGACUGGUg-GAGCUGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 38996 | 0.69 | 0.546329 |
Target: 5'- gCgUUGGUGaaGUUGGCCAgCUCGGCu -3' miRNA: 3'- -GgAGCCAUgaCGACUGGUgGAGCUGc -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 36687 | 0.69 | 0.546328 |
Target: 5'- aCCUCGcUGgaGCUGAuCCGCCgCGACc -3' miRNA: 3'- -GGAGCcAUgaCGACU-GGUGGaGCUGc -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 34567 | 0.67 | 0.665993 |
Target: 5'- gCCUUGGccagGCUccagaacgcaucGuCCACCUCGACGg -3' miRNA: 3'- -GGAGCCaugaCGA------------CuGGUGGAGCUGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 33047 | 0.75 | 0.256171 |
Target: 5'- --cUGGUGCUGCUGGCCACCgcCGcCGc -3' miRNA: 3'- ggaGCCAUGACGACUGGUGGa-GCuGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 32818 | 1.1 | 0.00084 |
Target: 5'- aCCUCGGUACUGCUGACCACCUCGACGc -3' miRNA: 3'- -GGAGCCAUGACGACUGGUGGAGCUGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 30702 | 0.68 | 0.615673 |
Target: 5'- --cCGGUcacagcguucuucacGCUGCUGGCCAguuCCUUGGCc -3' miRNA: 3'- ggaGCCA---------------UGACGACUGGU---GGAGCUGc -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 30306 | 0.7 | 0.493928 |
Target: 5'- uCCUCGGUcGC-GUUGACCuuCUCGAUc -3' miRNA: 3'- -GGAGCCA-UGaCGACUGGugGAGCUGc -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 27063 | 0.7 | 0.493928 |
Target: 5'- gCCUgGGcGCUGCUGAUCcUCUCGuCGc -3' miRNA: 3'- -GGAgCCaUGACGACUGGuGGAGCuGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 24045 | 0.68 | 0.5678 |
Target: 5'- aCCUCGGUGCcgagGC-GGCUAUCcCGGCa -3' miRNA: 3'- -GGAGCCAUGa---CGaCUGGUGGaGCUGc -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 23944 | 0.77 | 0.182068 |
Target: 5'- gUCUCGGUGCgGCUcGGuCCACCUCGAa- -3' miRNA: 3'- -GGAGCCAUGaCGA-CU-GGUGGAGCUgc -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 20119 | 0.66 | 0.719893 |
Target: 5'- aCCUgGGUcgGC-GCUGAUgGCCgaCGACGu -3' miRNA: 3'- -GGAgCCA--UGaCGACUGgUGGa-GCUGC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 19092 | 0.72 | 0.350734 |
Target: 5'- gCgUCGGUGCUuccucGCUGACCccggucacgucaugGCCUCGAuCGa -3' miRNA: 3'- -GgAGCCAUGA-----CGACUGG--------------UGGAGCU-GC- -5' |
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7183 | 3' | -55.3 | NC_001900.1 | + | 18669 | 0.66 | 0.698514 |
Target: 5'- gCCgugCGGcGCUGCaGGCgCAgCUCGACc -3' miRNA: 3'- -GGa--GCCaUGACGaCUG-GUgGAGCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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