Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7183 | 3' | -55.3 | NC_001900.1 | + | 9417 | 0.67 | 0.644138 |
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Target: 5'- aCC-CGGUGCUGU---UCGCgCUCGACGc -3' miRNA: 3'- -GGaGCCAUGACGacuGGUG-GAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 30702 | 0.68 | 0.615673 |
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Target: 5'- --cCGGUcacagcguucuucacGCUGCUGGCCAguuCCUUGGCc -3' miRNA: 3'- ggaGCCA---------------UGACGACUGGU---GGAGCUGc -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 38996 | 0.69 | 0.546329 |
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Target: 5'- gCgUUGGUGaaGUUGGCCAgCUCGGCu -3' miRNA: 3'- -GgAGCCAUgaCGACUGGUgGAGCUGc -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 42945 | 0.71 | 0.434292 |
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Target: 5'- aCUCGGg---GUUGGCUGCCUCGAUc -3' miRNA: 3'- gGAGCCaugaCGACUGGUGGAGCUGc -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 19092 | 0.72 | 0.350734 |
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Target: 5'- gCgUCGGUGCUuccucGCUGACCccggucacgucaugGCCUCGAuCGa -3' miRNA: 3'- -GgAGCCAUGA-----CGACUGG--------------UGGAGCU-GC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 41847 | 0.76 | 0.225097 |
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Target: 5'- cCCUCGGUGaucggacGCUGGCCAagCUCGAUGa -3' miRNA: 3'- -GGAGCCAUga-----CGACUGGUg-GAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 32818 | 1.1 | 0.00084 |
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Target: 5'- aCCUCGGUACUGCUGACCACCUCGACGc -3' miRNA: 3'- -GGAGCCAUGACGACUGGUGGAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 46921 | 0.66 | 0.740928 |
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Target: 5'- cCCUCGGg--UGCguuucGACCGcgagaaagcCCUCGAUGg -3' miRNA: 3'- -GGAGCCaugACGa----CUGGU---------GGAGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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