Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7183 | 3' | -55.3 | NC_001900.1 | + | 15255 | 0.66 | 0.698514 |
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Target: 5'- --gUGGUcCUGCUGcACCAgUUCGAUGa -3' miRNA: 3'- ggaGCCAuGACGAC-UGGUgGAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 18669 | 0.66 | 0.698514 |
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Target: 5'- gCCgugCGGcGCUGCaGGCgCAgCUCGACc -3' miRNA: 3'- -GGa--GCCaUGACGaCUG-GUgGAGCUGc -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 17749 | 0.66 | 0.698514 |
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Target: 5'- cCCUCGGcACUGCgcuuACCACUgcgcugcaGGCGa -3' miRNA: 3'- -GGAGCCaUGACGac--UGGUGGag------CUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 3004 | 0.66 | 0.708171 |
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Target: 5'- cUCUCcGcacUGCUGCUGACCgaaggcgACCggCGACGg -3' miRNA: 3'- -GGAGcC---AUGACGACUGG-------UGGa-GCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 20119 | 0.66 | 0.719893 |
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Target: 5'- aCCUgGGUcgGC-GCUGAUgGCCgaCGACGu -3' miRNA: 3'- -GGAgCCA--UGaCGACUGgUGGa-GCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 42294 | 0.66 | 0.73046 |
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Target: 5'- gCUCGau-CUGCagGAUCAgCUCGGCGa -3' miRNA: 3'- gGAGCcauGACGa-CUGGUgGAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 46921 | 0.66 | 0.740928 |
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Target: 5'- cCCUCGGg--UGCguuucGACCGcgagaaagcCCUCGAUGg -3' miRNA: 3'- -GGAGCCaugACGa----CUGGU---------GGAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 42732 | 0.66 | 0.740928 |
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Target: 5'- uCCUCGaUGucGUUGACCAUCUCG-CGg -3' miRNA: 3'- -GGAGCcAUgaCGACUGGUGGAGCuGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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