Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7183 | 3' | -55.3 | NC_001900.1 | + | 27063 | 0.7 | 0.493928 |
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Target: 5'- gCCUgGGcGCUGCUGAUCcUCUCGuCGc -3' miRNA: 3'- -GGAgCCaUGACGACUGGuGGAGCuGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 43446 | 0.71 | 0.442974 |
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Target: 5'- gUCUCGGaucACUGCuUGACCACCcccgcgucauggaUCGGCc -3' miRNA: 3'- -GGAGCCa--UGACG-ACUGGUGG-------------AGCUGc -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 42945 | 0.71 | 0.434292 |
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Target: 5'- aCUCGGg---GUUGGCUGCCUCGAUc -3' miRNA: 3'- gGAGCCaugaCGACUGGUGGAGCUGc -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 19092 | 0.72 | 0.350734 |
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Target: 5'- gCgUCGGUGCUuccucGCUGACCccggucacgucaugGCCUCGAuCGa -3' miRNA: 3'- -GgAGCCAUGA-----CGACUGG--------------UGGAGCU-GC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 33047 | 0.75 | 0.256171 |
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Target: 5'- --cUGGUGCUGCUGGCCACCgcCGcCGc -3' miRNA: 3'- ggaGCCAUGACGACUGGUGGa-GCuGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 41847 | 0.76 | 0.225097 |
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Target: 5'- cCCUCGGUGaucggacGCUGGCCAagCUCGAUGa -3' miRNA: 3'- -GGAGCCAUga-----CGACUGGUg-GAGCUGC- -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 23944 | 0.77 | 0.182068 |
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Target: 5'- gUCUCGGUGCgGCUcGGuCCACCUCGAa- -3' miRNA: 3'- -GGAGCCAUGaCGA-CU-GGUGGAGCUgc -5' |
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| 7183 | 3' | -55.3 | NC_001900.1 | + | 32818 | 1.1 | 0.00084 |
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Target: 5'- aCCUCGGUACUGCUGACCACCUCGACGc -3' miRNA: 3'- -GGAGCCAUGACGACUGGUGGAGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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