Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7184 | 5' | -59.4 | NC_001900.1 | + | 1174 | 0.69 | 0.302121 |
Target: 5'- gCGGCGGUGGAgCCGCCGgaAGC-GGCCgGg -3' miRNA: 3'- -GCUGUCGCCU-GGUGGC--UCGaCUGGgU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 3351 | 0.7 | 0.260461 |
Target: 5'- -cGCAGCGG-CCACguucuGCUGGCCCAc -3' miRNA: 3'- gcUGUCGCCuGGUGgcu--CGACUGGGU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 4495 | 0.69 | 0.309543 |
Target: 5'- aCGACGuUGGAgccuUCACCGAGgaGACCCc -3' miRNA: 3'- -GCUGUcGCCU----GGUGGCUCgaCUGGGu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 5573 | 0.73 | 0.172067 |
Target: 5'- gCGGagcaGGCGG-CgCACCGAGCUGGCCg- -3' miRNA: 3'- -GCUg---UCGCCuG-GUGGCUCGACUGGgu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 5819 | 0.73 | 0.167541 |
Target: 5'- -aGCGGaGGACCgagACUGGGCUGGCCCGg -3' miRNA: 3'- gcUGUCgCCUGG---UGGCUCGACUGGGU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 6750 | 0.66 | 0.456365 |
Target: 5'- cCGuCGGUcgagaaGGGugUCGCCGAGCUGAUCCu -3' miRNA: 3'- -GCuGUCG------CCU--GGUGGCUCGACUGGGu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 6828 | 0.65 | 0.493004 |
Target: 5'- gCGAUGGCGGguuacucgcagggaGCCAUCGuGGUUGGCCa- -3' miRNA: 3'- -GCUGUCGCC--------------UGGUGGC-UCGACUGGgu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 6983 | 0.7 | 0.253991 |
Target: 5'- uGACAGUGGAuCCAcCCGGucGCUGcCCCu -3' miRNA: 3'- gCUGUCGCCU-GGU-GGCU--CGACuGGGu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 8923 | 0.68 | 0.373887 |
Target: 5'- gCGGCAGCGGuCCuGCUGGacgcggcuGCUGGCCa- -3' miRNA: 3'- -GCUGUCGCCuGG-UGGCU--------CGACUGGgu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 10587 | 0.66 | 0.446715 |
Target: 5'- uCGAC-GCGauuACCGCUGAGCUGugCgGg -3' miRNA: 3'- -GCUGuCGCc--UGGUGGCUCGACugGgU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 19319 | 0.67 | 0.427762 |
Target: 5'- aCGGCGGUgGGGCCaacacggugGCCGAGCaGACUgGg -3' miRNA: 3'- -GCUGUCG-CCUGG---------UGGCUCGaCUGGgU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 21587 | 0.66 | 0.489982 |
Target: 5'- cCGACGGCcGGCUcaagGCCGugcggcucggccccaGGCUGAUCCGc -3' miRNA: 3'- -GCUGUCGcCUGG----UGGC---------------UCGACUGGGU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 22876 | 0.71 | 0.21791 |
Target: 5'- aGA-AGUGGAUCGCCGAGCgaGACCUc -3' miRNA: 3'- gCUgUCGCCUGGUGGCUCGa-CUGGGu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 22962 | 0.66 | 0.485966 |
Target: 5'- gCGACAcccCGG-UCGCCGAGaUGACCCc -3' miRNA: 3'- -GCUGUc--GCCuGGUGGCUCgACUGGGu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 23178 | 0.66 | 0.475995 |
Target: 5'- cCGGCcgAGCaGAUCGgUGAGCUGGCCg- -3' miRNA: 3'- -GCUG--UCGcCUGGUgGCUCGACUGGgu -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 23313 | 0.66 | 0.475995 |
Target: 5'- aCGGCGGUcGGCC-CCG-GCUGGgCCGa -3' miRNA: 3'- -GCUGUCGcCUGGuGGCuCGACUgGGU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 24896 | 0.67 | 0.409293 |
Target: 5'- cCGACA-UGGugUACCGAGaccgGACCUAc -3' miRNA: 3'- -GCUGUcGCCugGUGGCUCga--CUGGGU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 25916 | 0.69 | 0.309543 |
Target: 5'- aCGACGGUcuGGuCCucgcCCGGGCUGcGCCCGu -3' miRNA: 3'- -GCUGUCG--CCuGGu---GGCUCGAC-UGGGU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 26245 | 0.68 | 0.382542 |
Target: 5'- -aACAGCGGACCuucGCUGAGCgcgUCCAu -3' miRNA: 3'- gcUGUCGCCUGG---UGGCUCGacuGGGU- -5' |
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7184 | 5' | -59.4 | NC_001900.1 | + | 28003 | 0.73 | 0.172067 |
Target: 5'- aCGACGuGCGGAgCCcUCGGGCUGguGCCCAg -3' miRNA: 3'- -GCUGU-CGCCU-GGuGGCUCGAC--UGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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