Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7204 | 5' | -58.1 | NC_001900.1 | + | 21083 | 0.66 | 0.537599 |
Target: 5'- gUGCCCGCcggGGgCGGCGUCAacGUGcCGAu -3' miRNA: 3'- aACGGGCG---CUgGCUGUAGU--CGCuGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 24034 | 0.66 | 0.537599 |
Target: 5'- -aGCCCGCG-CCaACcUCGGUGcCGAg -3' miRNA: 3'- aaCGGGCGCuGGcUGuAGUCGCuGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 23590 | 0.66 | 0.527027 |
Target: 5'- -cGCCUaCGGUCGGCAUCAcCGACGGg -3' miRNA: 3'- aaCGGGcGCUGGCUGUAGUcGCUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 34621 | 0.66 | 0.516533 |
Target: 5'- gUGCCCGCGAagaacCCGgACGg-GGCGugGc -3' miRNA: 3'- aACGGGCGCU-----GGC-UGUagUCGCugCu -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 12758 | 0.66 | 0.516533 |
Target: 5'- -cGCCCGU--CCGACcauccUCAGCGACc- -3' miRNA: 3'- aaCGGGCGcuGGCUGu----AGUCGCUGcu -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 40316 | 0.66 | 0.516533 |
Target: 5'- -aGCCgcUGCGGCCGugGUUGGCcGCGu -3' miRNA: 3'- aaCGG--GCGCUGGCugUAGUCGcUGCu -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 13162 | 0.66 | 0.495806 |
Target: 5'- -cGCCCaGCGcCUGAUcgCGGCuGGCGGu -3' miRNA: 3'- aaCGGG-CGCuGGCUGuaGUCG-CUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 29227 | 0.66 | 0.485585 |
Target: 5'- -aGUCCGCG-UCGAagguCAUCcgacGGCGACGAg -3' miRNA: 3'- aaCGGGCGCuGGCU----GUAG----UCGCUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 3349 | 0.67 | 0.469443 |
Target: 5'- -gGCCUGCGGgCGACcuguUCcucgaacuggagaccGGCGGCGAg -3' miRNA: 3'- aaCGGGCGCUgGCUGu---AG---------------UCGCUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 42373 | 0.67 | 0.46545 |
Target: 5'- cUGCCCGCG-UCGAUcgCucgAGCGACc- -3' miRNA: 3'- aACGGGCGCuGGCUGuaG---UCGCUGcu -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 13000 | 0.67 | 0.46545 |
Target: 5'- -cGCUC-UGACCaacGugGUCAGCGGCGGc -3' miRNA: 3'- aaCGGGcGCUGG---CugUAGUCGCUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 31206 | 0.67 | 0.462467 |
Target: 5'- -gGgCCGaCGACCuccuucuuggccguGcCGUCAGCGACGAa -3' miRNA: 3'- aaCgGGC-GCUGG--------------CuGUAGUCGCUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 2263 | 0.67 | 0.455547 |
Target: 5'- -gGCCa--GGUCGAgGUCAGCGACGAg -3' miRNA: 3'- aaCGGgcgCUGGCUgUAGUCGCUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 14500 | 0.67 | 0.445758 |
Target: 5'- cUGaCCCGa-GCCGcCAUCGGCGGCa- -3' miRNA: 3'- aAC-GGGCgcUGGCuGUAGUCGCUGcu -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 1778 | 0.67 | 0.445758 |
Target: 5'- -gGUgCGUGACCGGgucacugugacCAUCGGCGACu- -3' miRNA: 3'- aaCGgGCGCUGGCU-----------GUAGUCGCUGcu -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 11659 | 0.67 | 0.436088 |
Target: 5'- -aGCCCGaCGAggUCGuccACAUCGGCGACc- -3' miRNA: 3'- aaCGGGC-GCU--GGC---UGUAGUCGCUGcu -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 32109 | 0.67 | 0.436088 |
Target: 5'- -aGCgCCGaacGCUGACAgCAGCGACGGc -3' miRNA: 3'- aaCG-GGCgc-UGGCUGUaGUCGCUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 22257 | 0.67 | 0.436088 |
Target: 5'- -gGCCCaGCGaugGCCGAgGUCguggAGUGACGGa -3' miRNA: 3'- aaCGGG-CGC---UGGCUgUAG----UCGCUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 31049 | 0.67 | 0.433211 |
Target: 5'- -gGCaCGaUGACCGGCAUCAGCuuggugaacgccugGACGAu -3' miRNA: 3'- aaCGgGC-GCUGGCUGUAGUCG--------------CUGCU- -5' |
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7204 | 5' | -58.1 | NC_001900.1 | + | 32162 | 0.67 | 0.42654 |
Target: 5'- -aGCCgacaaGCGGCgCGGCGaUGGCGACGAu -3' miRNA: 3'- aaCGGg----CGCUG-GCUGUaGUCGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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