Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7253 | 3' | -62.7 | NC_001900.1 | + | 15548 | 0.69 | 0.189189 |
Target: 5'- cGGCcUCUCG-CCGGGCGgUG-GAGCCu -3' miRNA: 3'- -UCGcGGAGCgGGUCCGCgACaCUCGG- -5' |
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7253 | 3' | -62.7 | NC_001900.1 | + | 3118 | 0.7 | 0.169482 |
Target: 5'- cAGCGCCUCGCCaAGGCGagcggcgggggGUGAugGUCc -3' miRNA: 3'- -UCGCGGAGCGGgUCCGCga---------CACU--CGG- -5' |
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7253 | 3' | -62.7 | NC_001900.1 | + | 48622 | 0.71 | 0.13771 |
Target: 5'- aGGCuGCUcguuaugUGCCCgguauGGGUGCUGUGAGCUg -3' miRNA: 3'- -UCG-CGGa------GCGGG-----UCCGCGACACUCGG- -5' |
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7253 | 3' | -62.7 | NC_001900.1 | + | 68 | 1.11 | 0.0001 |
Target: 5'- gAGCGCCUCGCCCAGGCGCUGUGAGCCa -3' miRNA: 3'- -UCGCGGAGCGGGUCCGCGACACUCGG- -5' |
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7253 | 3' | -62.7 | NC_001900.1 | + | 267 | 0.66 | 0.319701 |
Target: 5'- aGGCGgCUCGCUaagagcgccuuuCGGGCGCUccUGAGUa -3' miRNA: 3'- -UCGCgGAGCGG------------GUCCGCGAc-ACUCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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