Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7253 | 3' | -62.7 | NC_001900.1 | + | 2656 | 0.66 | 0.297544 |
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Target: 5'- aGGUGCCU-GCUCcacccaggAGGUGaCUGUGGGCa -3' miRNA: 3'- -UCGCGGAgCGGG--------UCCGC-GACACUCGg -5' |
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| 7253 | 3' | -62.7 | NC_001900.1 | + | 30569 | 0.66 | 0.304795 |
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Target: 5'- cAGUGCgCUgGCaCCGcuGGUGCccgacGUGAGCCg -3' miRNA: 3'- -UCGCG-GAgCG-GGU--CCGCGa----CACUCGG- -5' |
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| 7253 | 3' | -62.7 | NC_001900.1 | + | 24873 | 0.66 | 0.304795 |
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Target: 5'- cGUGCCacCGCCCgagaAGGUGgCUGUGGuGUCg -3' miRNA: 3'- uCGCGGa-GCGGG----UCCGC-GACACU-CGG- -5' |
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| 7253 | 3' | -62.7 | NC_001900.1 | + | 33786 | 0.66 | 0.31218 |
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Target: 5'- cAGCuCCUgGCguuCCAGGCGCucuaccagacgUGcUGGGCCg -3' miRNA: 3'- -UCGcGGAgCG---GGUCCGCG-----------AC-ACUCGG- -5' |
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| 7253 | 3' | -62.7 | NC_001900.1 | + | 267 | 0.66 | 0.319701 |
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Target: 5'- aGGCGgCUCGCUaagagcgccuuuCGGGCGCUccUGAGUa -3' miRNA: 3'- -UCGCgGAGCGG------------GUCCGCGAc-ACUCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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