Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7254 | 3' | -53 | NC_001900.1 | + | 28064 | 0.66 | 0.784576 |
Target: 5'- cGUGAUcuUCGuGCCCGcCGGGGGCgGc -3' miRNA: 3'- uCAUUAuuGGC-CGGGUuGUUCCCGgC- -5' |
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7254 | 3' | -53 | NC_001900.1 | + | 37275 | 0.66 | 0.79467 |
Target: 5'- cGUcGUAcaCGGCCC-GCAgcGGGGCCa -3' miRNA: 3'- uCAuUAUugGCCGGGuUGU--UCCCGGc -5' |
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7254 | 3' | -53 | NC_001900.1 | + | 11276 | 0.66 | 0.79467 |
Target: 5'- cGUGGagAACUGGCCCGGaaa-GGCCGc -3' miRNA: 3'- uCAUUa-UUGGCCGGGUUguucCCGGC- -5' |
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7254 | 3' | -53 | NC_001900.1 | + | 34862 | 0.66 | 0.804585 |
Target: 5'- --gAGgcACCGGCCCAGa---GGCCGu -3' miRNA: 3'- ucaUUauUGGCCGGGUUguucCCGGC- -5' |
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7254 | 3' | -53 | NC_001900.1 | + | 5564 | 0.66 | 0.814309 |
Target: 5'- cGUGAUcaugauccagGACCGGCCCuuguAGaGCCGa -3' miRNA: 3'- uCAUUA----------UUGGCCGGGuuguUCcCGGC- -5' |
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7254 | 3' | -53 | NC_001900.1 | + | 16144 | 0.66 | 0.814309 |
Target: 5'- -----cGAUCaGCUCGGCGAGGGCgCGg -3' miRNA: 3'- ucauuaUUGGcCGGGUUGUUCCCG-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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