Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7254 | 3' | -53 | NC_001900.1 | + | 15615 | 0.69 | 0.631255 |
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Target: 5'- ------cACC-GCCCGGCGAGaGGCCGa -3' miRNA: 3'- ucauuauUGGcCGGGUUGUUC-CCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 30795 | 0.69 | 0.597331 |
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Target: 5'- uGGUGGacccCCGGCCUuuC-GGGGCCGg -3' miRNA: 3'- -UCAUUauu-GGCCGGGuuGuUCCCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 21379 | 0.7 | 0.540435 |
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Target: 5'- gGGUucagGACCGcauccaucagaccGCCCAGCGGGGGCa- -3' miRNA: 3'- -UCAuua-UUGGC-------------CGGGUUGUUCCCGgc -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 5847 | 0.71 | 0.498182 |
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Target: 5'- gAGUcAGUAGuuGgucGCCCAcaGCGAGGGCCa -3' miRNA: 3'- -UCA-UUAUUggC---CGGGU--UGUUCCCGGc -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 23397 | 0.85 | 0.064287 |
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Target: 5'- cGGUAuagAACuCGGCCCAGCcGGGGCCGa -3' miRNA: 3'- -UCAUua-UUG-GCCGGGUUGuUCCCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 16144 | 0.66 | 0.814309 |
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Target: 5'- -----cGAUCaGCUCGGCGAGGGCgCGg -3' miRNA: 3'- ucauuaUUGGcCGGGUUGUUCCCG-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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