Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7254 | 3' | -53 | NC_001900.1 | + | 26661 | 0.7 | 0.552578 |
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Target: 5'- aGGUGGUuuuuagcccgagGACCGGaucCCCGAgaGGGGGCCGc -3' miRNA: 3'- -UCAUUA------------UUGGCC---GGGUUg-UUCCCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 28064 | 0.66 | 0.784576 |
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Target: 5'- cGUGAUcuUCGuGCCCGcCGGGGGCgGc -3' miRNA: 3'- uCAUUAuuGGC-CGGGUuGUUCCCGgC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 30795 | 0.69 | 0.597331 |
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Target: 5'- uGGUGGacccCCGGCCUuuC-GGGGCCGg -3' miRNA: 3'- -UCAUUauu-GGCCGGGuuGuUCCCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 32737 | 0.69 | 0.642579 |
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Target: 5'- cGUAGUcgguGCCuGCCCAGCucGGGUCu -3' miRNA: 3'- uCAUUAu---UGGcCGGGUUGuuCCCGGc -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 34862 | 0.66 | 0.804585 |
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Target: 5'- --gAGgcACCGGCCCAGa---GGCCGu -3' miRNA: 3'- ucaUUauUGGCCGGGUUguucCCGGC- -5' |
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| 7254 | 3' | -53 | NC_001900.1 | + | 37275 | 0.66 | 0.79467 |
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Target: 5'- cGUcGUAcaCGGCCC-GCAgcGGGGCCa -3' miRNA: 3'- uCAuUAUugGCCGGGuUGU--UCCCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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