Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7262 | 3' | -52.2 | NC_001900.1 | + | 44771 | 0.66 | 0.858336 |
Target: 5'- -gGCCAgCGGCcUGGcuGCCGCAGGGGa -3' miRNA: 3'- gaCGGUgGCUGcAUC--UGGUGUUCUUc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 29760 | 0.66 | 0.858336 |
Target: 5'- --cCCACCGcCGUAGACC-CGGccGAAGu -3' miRNA: 3'- gacGGUGGCuGCAUCUGGuGUU--CUUC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 46006 | 0.66 | 0.858336 |
Target: 5'- -aGCgCGaaGGCGaGGACCGCGAGAu- -3' miRNA: 3'- gaCG-GUggCUGCaUCUGGUGUUCUuc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 8264 | 0.66 | 0.858336 |
Target: 5'- cCUGCCACuCGACGUAcgccuucucggcGACCGucucggugcCGGGAu- -3' miRNA: 3'- -GACGGUG-GCUGCAU------------CUGGU---------GUUCUuc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 33949 | 0.66 | 0.849683 |
Target: 5'- -cGCgGCUGACGgguACCACGucgAGAAGg -3' miRNA: 3'- gaCGgUGGCUGCaucUGGUGU---UCUUC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 24061 | 0.66 | 0.840788 |
Target: 5'- gUGCCGCCGuuGgGGAUCuGCAGGAu- -3' miRNA: 3'- gACGGUGGCugCaUCUGG-UGUUCUuc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 25588 | 0.66 | 0.840788 |
Target: 5'- gCUGCCcCCG-CGUGGuggucACCAgGAGAGc -3' miRNA: 3'- -GACGGuGGCuGCAUC-----UGGUgUUCUUc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 23655 | 0.66 | 0.840788 |
Target: 5'- -gGCUACCGGCGUAgaggcucuGACCACu----- -3' miRNA: 3'- gaCGGUGGCUGCAU--------CUGGUGuucuuc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 15110 | 0.66 | 0.840788 |
Target: 5'- -aGCCACCGgcuggucgaGCGUcGGCCACAccAGccGAGg -3' miRNA: 3'- gaCGGUGGC---------UGCAuCUGGUGU--UC--UUC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 41151 | 0.66 | 0.839886 |
Target: 5'- -gGUCACCGGCcUGGcCCGCAacgucucGGAGGa -3' miRNA: 3'- gaCGGUGGCUGcAUCuGGUGU-------UCUUC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 3067 | 0.66 | 0.831661 |
Target: 5'- --cCCGCCGACcc-GACCGgCGGGAAGg -3' miRNA: 3'- gacGGUGGCUGcauCUGGU-GUUCUUC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 6734 | 0.66 | 0.831661 |
Target: 5'- -gGCCuucCCGAUGUGG-CCGucgguCGAGAAGg -3' miRNA: 3'- gaCGGu--GGCUGCAUCuGGU-----GUUCUUC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 1680 | 0.66 | 0.830736 |
Target: 5'- -cGCCACCGGCcgagaagccGACCucgaacaGCGAGGAGa -3' miRNA: 3'- gaCGGUGGCUGcau------CUGG-------UGUUCUUC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 23226 | 0.66 | 0.826078 |
Target: 5'- uCUGCCAUCGcCGagcugcaggcucgccUGGACgCACAGGAc- -3' miRNA: 3'- -GACGGUGGCuGC---------------AUCUG-GUGUUCUuc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 42827 | 0.66 | 0.822311 |
Target: 5'- cCUGgUGCCGAUGgacgAGGuCCGCGAGAu- -3' miRNA: 3'- -GACgGUGGCUGCa---UCU-GGUGUUCUuc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 23876 | 0.67 | 0.81275 |
Target: 5'- -aGCCgACCGGagccCGUcgucGGACCACAGGGc- -3' miRNA: 3'- gaCGG-UGGCU----GCA----UCUGGUGUUCUuc -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 35768 | 0.67 | 0.81275 |
Target: 5'- -cGCCGCCGACGUAcuGCUcCAGGucGu -3' miRNA: 3'- gaCGGUGGCUGCAUc-UGGuGUUCuuC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 32667 | 0.67 | 0.802987 |
Target: 5'- --aCCACauccuCGACGUGGGCCAUGAGucGg -3' miRNA: 3'- gacGGUG-----GCUGCAUCUGGUGUUCuuC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 31292 | 0.67 | 0.793035 |
Target: 5'- -cGUCGCUGACGgcacGGCCAagaAGGAGGu -3' miRNA: 3'- gaCGGUGGCUGCau--CUGGUg--UUCUUC- -5' |
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7262 | 3' | -52.2 | NC_001900.1 | + | 23394 | 0.67 | 0.793035 |
Target: 5'- -gGCCaucagGCCGA-GUGGACCGacCAGGGAGc -3' miRNA: 3'- gaCGG-----UGGCUgCAUCUGGU--GUUCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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