Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7285 | 5' | -59.5 | NC_001900.1 | + | 27469 | 0.71 | 0.235371 |
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Target: 5'- gGGCcGAGcCGCaCGGCCuuGAgccGGCCGUCGg -3' miRNA: 3'- -CCGaCUC-GCG-GCUGGu-CU---CCGGUAGC- -5' |
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| 7285 | 5' | -59.5 | NC_001900.1 | + | 23401 | 0.71 | 0.217911 |
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Target: 5'- aGGCcGAGUGgaCCGACCAGGGaGCCAc-- -3' miRNA: 3'- -CCGaCUCGC--GGCUGGUCUC-CGGUagc -5' |
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| 7285 | 5' | -59.5 | NC_001900.1 | + | 36318 | 0.72 | 0.20157 |
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Target: 5'- gGGCUccaagaacGAGCGCC-ACgCAGAGGCgAUCa -3' miRNA: 3'- -CCGA--------CUCGCGGcUG-GUCUCCGgUAGc -5' |
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| 7285 | 5' | -59.5 | NC_001900.1 | + | 23883 | 0.72 | 0.196364 |
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Target: 5'- cGCcGAuaGCCGACCGGAGcCCGUCGu -3' miRNA: 3'- cCGaCUcgCGGCUGGUCUCcGGUAGC- -5' |
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| 7285 | 5' | -59.5 | NC_001900.1 | + | 5392 | 0.73 | 0.158809 |
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Target: 5'- gGGUUGGuGCaGUCGGCCuggauGAGGCCGUCa -3' miRNA: 3'- -CCGACU-CG-CGGCUGGu----CUCCGGUAGc -5' |
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| 7285 | 5' | -59.5 | NC_001900.1 | + | 27177 | 0.74 | 0.15049 |
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Target: 5'- aGGCUGGGCGUgGGCCAcGuuGCCcgCGa -3' miRNA: 3'- -CCGACUCGCGgCUGGU-CucCGGuaGC- -5' |
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| 7285 | 5' | -59.5 | NC_001900.1 | + | 11619 | 0.77 | 0.09177 |
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Target: 5'- gGGUcGGGuCGCCGGCCGGAGuGCCuUCGg -3' miRNA: 3'- -CCGaCUC-GCGGCUGGUCUC-CGGuAGC- -5' |
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| 7285 | 5' | -59.5 | NC_001900.1 | + | 9277 | 1.11 | 0.000237 |
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Target: 5'- aGGCUGAGCGCCGACCAGAGGCCAUCGg -3' miRNA: 3'- -CCGACUCGCGGCUGGUCUCCGGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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