Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
760 | 5' | -52.4 | NC_000852.3 | + | 61612 | 0.67 | 0.998002 |
Target: 5'- aGGAGAugCCaCGAAggcuaaaagcGUAUUGGguugGGAACCc -3' miRNA: 3'- -CCUCUugGG-GCUU----------CAUGGCC----UCUUGG- -5' |
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760 | 5' | -52.4 | NC_000852.3 | + | 171190 | 0.66 | 0.998578 |
Target: 5'- uGGAGAaaugcGCCCUGAAuucGUACCuGAaacGACCa -3' miRNA: 3'- -CCUCU-----UGGGGCUU---CAUGGcCUc--UUGG- -5' |
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760 | 5' | -52.4 | NC_000852.3 | + | 235983 | 0.66 | 0.999006 |
Target: 5'- uGGAGAAgCCUGAGGUugagaaauagggACCGGu--GCa -3' miRNA: 3'- -CCUCUUgGGGCUUCA------------UGGCCucuUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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