miRNA: 3'- ccUCUUGGGGCu---UCaugGCCUCUUGG- -5'
miRNA: 3'- cCUCUUgGGGCUUCaUGGcCUCUUGG- -5'
miRNA: 3'- -CCUCUUgGGGCUUCA------------UGGCCucuUGg -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 760 | 5' | -52.4 | NC_000852.3 | + | 151467 | 0.72 | 0.959015 |
|
Target: 5'- -aAGAACCCCGcacuGGggaCGGAGAACUu -3' miRNA: 3'- ccUCUUGGGGCu---UCaugGCCUCUUGG- -5' |
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| 760 | 5' | -52.4 | NC_000852.3 | + | 284972 | 0.73 | 0.938969 |
|
Target: 5'- uGAGAA-CCCGAGG-ACCcGAGGACCc -3' miRNA: 3'- cCUCUUgGGGCUUCaUGGcCUCUUGG- -5' |
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| 760 | 5' | -52.4 | NC_000852.3 | + | 235983 | 0.66 | 0.999006 |
|
Target: 5'- uGGAGAAgCCUGAGGUugagaaauagggACCGGu--GCa -3' miRNA: 3'- -CCUCUUgGGGCUUCA------------UGGCCucuUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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