Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7633 | 5' | -55.1 | NC_001973.1 | + | 117979 | 0.66 | 0.948002 |
Target: 5'- cGGCCGugcgCGAauUCAUCGAGGagacgggcugcguguUCGAGa -3' miRNA: 3'- -CCGGCcucaGCU--AGUAGCUCC---------------AGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 79643 | 0.66 | 0.941879 |
Target: 5'- cGCCGG-GUCG-UCGUCGucGUCGc- -3' miRNA: 3'- cCGGCCuCAGCuAGUAGCucCAGCuc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 28961 | 0.66 | 0.941879 |
Target: 5'- gGGCCGGcAGUCGGgcggcgacgCGUUGuaGUCGGGg -3' miRNA: 3'- -CCGGCC-UCAGCUa--------GUAGCucCAGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 33463 | 0.67 | 0.936274 |
Target: 5'- aGUCGGAGaugagguggaaGAUC-UCGGGGUCGAa -3' miRNA: 3'- cCGGCCUCag---------CUAGuAGCUCCAGCUc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 151100 | 0.67 | 0.932352 |
Target: 5'- cGCCGGGGUCGu---UUGAGGUUa-- -3' miRNA: 3'- cCGGCCUCAGCuaguAGCUCCAGcuc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 85317 | 0.67 | 0.927242 |
Target: 5'- uGuuGGAG-CGGcUCAcguUCGAGGUCGGc -3' miRNA: 3'- cCggCCUCaGCU-AGU---AGCUCCAGCUc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 87539 | 0.67 | 0.927242 |
Target: 5'- cGGCCGGugccGGUCGAggccgCAUCGGcGGUUc-- -3' miRNA: 3'- -CCGGCC----UCAGCUa----GUAGCU-CCAGcuc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 7041 | 0.67 | 0.927242 |
Target: 5'- aGCUGGAcgaGUCGGUCGUgaaCGAgcugcgcgcccuGGUCGAGc -3' miRNA: 3'- cCGGCCU---CAGCUAGUA---GCU------------CCAGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 135909 | 0.67 | 0.916325 |
Target: 5'- gGGCCGGA--CGAUCGUCGcAGaG-CGGGc -3' miRNA: 3'- -CCGGCCUcaGCUAGUAGC-UC-CaGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 150933 | 0.68 | 0.910519 |
Target: 5'- cGCCGGGGUCGu---UgGAGGUCa-- -3' miRNA: 3'- cCGGCCUCAGCuaguAgCUCCAGcuc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 57213 | 0.68 | 0.885028 |
Target: 5'- gGGCCGucGaCGA-CGUCGAGGUCGc- -3' miRNA: 3'- -CCGGCcuCaGCUaGUAGCUCCAGCuc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 12146 | 0.69 | 0.870965 |
Target: 5'- uGCCGGGGUCa---AUCGAGuUCGAGc -3' miRNA: 3'- cCGGCCUCAGcuagUAGCUCcAGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 151384 | 0.69 | 0.85607 |
Target: 5'- cGGCCGGGGUCG-UC--UGAGGUUa-- -3' miRNA: 3'- -CCGGCCUCAGCuAGuaGCUCCAGcuc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 21192 | 0.69 | 0.85607 |
Target: 5'- cGGCCGGGGUCGAUCGgguUCaAGccCGAu -3' miRNA: 3'- -CCGGCCUCAGCUAGU---AGcUCcaGCUc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 130573 | 0.69 | 0.848325 |
Target: 5'- cGGCCGGGGUCGA---UCGAGuUCa-- -3' miRNA: 3'- -CCGGCCUCAGCUaguAGCUCcAGcuc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 12458 | 0.69 | 0.848325 |
Target: 5'- cGGCCGGGGUCGA---UCGAGuUCa-- -3' miRNA: 3'- -CCGGCCUCAGCUaguAGCUCcAGcuc -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 2833 | 0.7 | 0.832271 |
Target: 5'- cGGCCGGGGUCGAUC----GGGUucaagcccgaUGAGa -3' miRNA: 3'- -CCGGCCUCAGCUAGuagcUCCA----------GCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 2715 | 0.7 | 0.832271 |
Target: 5'- cGGCCGGGGUCGAUC----GGGUucaagcccgaUGAGa -3' miRNA: 3'- -CCGGCCUCAGCUAGuagcUCCA----------GCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 67853 | 0.7 | 0.798101 |
Target: 5'- cGCuCGGAGaCGGUCGUUGAGcgaGUUGAGg -3' miRNA: 3'- cCG-GCCUCaGCUAGUAGCUC---CAGCUC- -5' |
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7633 | 5' | -55.1 | NC_001973.1 | + | 90290 | 0.71 | 0.777366 |
Target: 5'- cGGCCGGuguucaaggcgcacAGUCGGUCGgcgggCGAGG-CGGu -3' miRNA: 3'- -CCGGCC--------------UCAGCUAGUa----GCUCCaGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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