Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7643 | 3' | -53.5 | NC_001973.1 | + | 145127 | 0.67 | 0.960199 |
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Target: 5'- uUGAC-GGCCgagcaGGCUCuGGACucGGUCCg -3' miRNA: 3'- gACUGuUUGGa----UCGAG-CCUGc-CCAGG- -5' |
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| 7643 | 3' | -53.5 | NC_001973.1 | + | 131777 | 0.67 | 0.963676 |
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Target: 5'- uUGACGAcggcgGCC-GGCUCGGugaaGGGgCCg -3' miRNA: 3'- gACUGUU-----UGGaUCGAGCCug--CCCaGG- -5' |
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| 7643 | 3' | -53.5 | NC_001973.1 | + | 153342 | 0.66 | 0.968179 |
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Target: 5'- cCUGAaaaucGCCaagAGCgaggucgauuucgacUCGGACGGGUCg -3' miRNA: 3'- -GACUguu--UGGa--UCG---------------AGCCUGCCCAGg -5' |
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| 7643 | 3' | -53.5 | NC_001973.1 | + | 21530 | 0.66 | 0.969982 |
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Target: 5'- -cGACGuGCCUgaucucGGCgUCGGGCGcgccguGGUCCc -3' miRNA: 3'- gaCUGUuUGGA------UCG-AGCCUGC------CCAGG- -5' |
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| 7643 | 3' | -53.5 | NC_001973.1 | + | 140973 | 0.66 | 0.972823 |
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Target: 5'- -cGACAucACCUu-CUCGGACGaGG-CCg -3' miRNA: 3'- gaCUGUu-UGGAucGAGCCUGC-CCaGG- -5' |
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| 7643 | 3' | -53.5 | NC_001973.1 | + | 59535 | 0.66 | 0.977912 |
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Target: 5'- -aGACGAugC--GCUUGGACGcGG-CCa -3' miRNA: 3'- gaCUGUUugGauCGAGCCUGC-CCaGG- -5' |
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| 7643 | 3' | -53.5 | NC_001973.1 | + | 64104 | 0.66 | 0.977912 |
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Target: 5'- --aACAGGCCgacGGCUCG-ACGGGgcggugCCg -3' miRNA: 3'- gacUGUUUGGa--UCGAGCcUGCCCa-----GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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