Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7645 | 3' | -57.2 | NC_001973.1 | + | 8803 | 0.66 | 0.872977 |
Target: 5'- cGGAAcccGCGGCgcgaagacacgaCGAGCCCGC-CCg- -3' miRNA: 3'- -UCUUuaaCGCCG------------GCUCGGGCGaGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 45223 | 0.66 | 0.865605 |
Target: 5'- cGGGccGcgUGCuGCCG-GCCCGCcaCCUGg -3' miRNA: 3'- -UCU--UuaACGcCGGCuCGGGCGa-GGAC- -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 98059 | 0.66 | 0.858024 |
Target: 5'- aAGAGcgcGUaGUGGCCGuAGCCgCGCUCgUa -3' miRNA: 3'- -UCUU---UAaCGCCGGC-UCGG-GCGAGgAc -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 83644 | 0.66 | 0.858024 |
Target: 5'- cGAAAUgaacgaagcgcUGCGGC--GGCCCGCUCgCUu -3' miRNA: 3'- uCUUUA-----------ACGCCGgcUCGGGCGAG-GAc -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 141790 | 0.66 | 0.857254 |
Target: 5'- gAGGAAUUGCGcccGCCGAacgcuaacgcgacGcCCCGCUUCa- -3' miRNA: 3'- -UCUUUAACGC---CGGCU-------------C-GGGCGAGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 26255 | 0.66 | 0.84226 |
Target: 5'- cGAccgUGCGGCaCGAGCguCCGCUuCCa- -3' miRNA: 3'- uCUuuaACGCCG-GCUCG--GGCGA-GGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 83845 | 0.66 | 0.841451 |
Target: 5'- gAGAGAgaGCGGCacgcgagCGAGCUCGC-CCg- -3' miRNA: 3'- -UCUUUaaCGCCG-------GCUCGGGCGaGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 33333 | 0.66 | 0.83409 |
Target: 5'- cGGGucacGUUGCGGCCGcggaugauGCCCGUgCCg- -3' miRNA: 3'- -UCUu---UAACGCCGGCu-------CGGGCGaGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 45967 | 0.67 | 0.825738 |
Target: 5'- ------gGCGGCCGGcgcGCUCGC-CCUGc -3' miRNA: 3'- ucuuuaaCGCCGGCU---CGGGCGaGGAC- -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 48405 | 0.67 | 0.825738 |
Target: 5'- ------gGC-GCUGAGCCUGCUCCa- -3' miRNA: 3'- ucuuuaaCGcCGGCUCGGGCGAGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 83041 | 0.67 | 0.808517 |
Target: 5'- cGAGGcgGCGcGCCcgucGAGCCCGCggCCUc -3' miRNA: 3'- uCUUUaaCGC-CGG----CUCGGGCGa-GGAc -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 155323 | 0.67 | 0.799665 |
Target: 5'- ------cGCucCCGAGCCCGCUCCc- -3' miRNA: 3'- ucuuuaaCGccGGCUCGGGCGAGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 77367 | 0.67 | 0.790661 |
Target: 5'- ------cGCGGCCGcgcGCCCGCgcgCCg- -3' miRNA: 3'- ucuuuaaCGCCGGCu--CGGGCGa--GGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 36699 | 0.67 | 0.790661 |
Target: 5'- ------cGCGGCCGucGUCCGCUUCg- -3' miRNA: 3'- ucuuuaaCGCCGGCu-CGGGCGAGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 28295 | 0.67 | 0.790661 |
Target: 5'- ------gGCGGCCGAGCgCCGCgUCg-- -3' miRNA: 3'- ucuuuaaCGCCGGCUCG-GGCG-AGgac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 160835 | 0.68 | 0.762836 |
Target: 5'- cGGAGUcGaucaucgaGGUCGAGCCCGCUCg-- -3' miRNA: 3'- uCUUUAaCg-------CCGGCUCGGGCGAGgac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 157895 | 0.68 | 0.733975 |
Target: 5'- cGGAGGUcGCGGCCGccgcGCCCGCg---- -3' miRNA: 3'- -UCUUUAaCGCCGGCu---CGGGCGaggac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 155203 | 0.69 | 0.714281 |
Target: 5'- -cGAGUUcGCucCCGAGCCCGCUCCc- -3' miRNA: 3'- ucUUUAA-CGccGGCUCGGGCGAGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 145123 | 0.69 | 0.714281 |
Target: 5'- -cAAGUUGaCGGCCGAGCagGCUCUg- -3' miRNA: 3'- ucUUUAAC-GCCGGCUCGggCGAGGac -5' |
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7645 | 3' | -57.2 | NC_001973.1 | + | 117801 | 0.69 | 0.704324 |
Target: 5'- ---cGUUGC-GCCG-GCCUGUUCCUGa -3' miRNA: 3'- ucuuUAACGcCGGCuCGGGCGAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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