Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7652 | 5' | -57.7 | NC_001973.1 | + | 138635 | 0.66 | 0.85693 |
Target: 5'- uGCGcCAGCGcgggcGGCACGgGCUuuUGGAa -3' miRNA: 3'- -CGCaGUCGU-----CCGUGUgCGAcgACCUc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 64866 | 0.66 | 0.85693 |
Target: 5'- cCGUCccuGCGGGCuCGCGCccgaagUGCUGGc- -3' miRNA: 3'- cGCAGu--CGUCCGuGUGCG------ACGACCuc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 40014 | 0.66 | 0.849146 |
Target: 5'- cCGcCAGCAGGCcgaacuCGCGCUGCa---- -3' miRNA: 3'- cGCaGUCGUCCGu-----GUGCGACGaccuc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 4197 | 0.66 | 0.841168 |
Target: 5'- cCG-CAGCuggAGGUGCAUGC-GCUGGAa -3' miRNA: 3'- cGCaGUCG---UCCGUGUGCGaCGACCUc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 130778 | 0.66 | 0.824657 |
Target: 5'- aGC-UCGGCGGGCGCguaggauuuuACGCgucgGCcGGGGu -3' miRNA: 3'- -CGcAGUCGUCCGUG----------UGCGa---CGaCCUC- -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 21064 | 0.66 | 0.824657 |
Target: 5'- aGC-UCGGCGGGCGCguaggaucuuACGCgucgGCcGGGGu -3' miRNA: 3'- -CGcAGUCGUCCGUG----------UGCGa---CGaCCUC- -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 131491 | 0.66 | 0.824657 |
Target: 5'- -gGUCGGCGGGCccgcCGCGC-GCcGGAu -3' miRNA: 3'- cgCAGUCGUCCGu---GUGCGaCGaCCUc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 92617 | 0.66 | 0.816139 |
Target: 5'- cGCGcCAGCAGGCGUAaGC-GCUGGu- -3' miRNA: 3'- -CGCaGUCGUCCGUGUgCGaCGACCuc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 17049 | 0.67 | 0.807456 |
Target: 5'- gGCgGUCGGCgcuuGGGCggugaGCAUGCUGUUGGc- -3' miRNA: 3'- -CG-CAGUCG----UCCG-----UGUGCGACGACCuc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 16184 | 0.67 | 0.797724 |
Target: 5'- cGUGUCGgcGCAGGCcgcuagaGCGCGCUGCg---- -3' miRNA: 3'- -CGCAGU--CGUCCG-------UGUGCGACGaccuc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 81051 | 0.67 | 0.771241 |
Target: 5'- cGCGUCGaCGGGCGUGCGCUucaGUUGGGa -3' miRNA: 3'- -CGCAGUcGUCCGUGUGCGA---CGACCUc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 131871 | 0.67 | 0.771241 |
Target: 5'- cGCaaugCGGCAGGCACagacGCGCUcGgUGGAa -3' miRNA: 3'- -CGca--GUCGUCCGUG----UGCGA-CgACCUc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 53563 | 0.67 | 0.768439 |
Target: 5'- uCGUCGGCcaugucugcgacugAGGCGCGCGCcgGCgccGAGg -3' miRNA: 3'- cGCAGUCG--------------UCCGUGUGCGa-CGac-CUC- -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 95399 | 0.68 | 0.752365 |
Target: 5'- cCGggCGGCAagGGCGCcaaccCGCUGCUGGGc -3' miRNA: 3'- cGCa-GUCGU--CCGUGu----GCGACGACCUc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 54958 | 0.68 | 0.752365 |
Target: 5'- --uUCAGCGGGCACGC-CgaugagGCcGGAGg -3' miRNA: 3'- cgcAGUCGUCCGUGUGcGa-----CGaCCUC- -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 160801 | 0.68 | 0.742765 |
Target: 5'- aGC-UCGGCGGGCGCguaggauuuuACGCgucgGCcGGAGu -3' miRNA: 3'- -CGcAGUCGUCCGUG----------UGCGa---CGaCCUC- -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 120789 | 0.68 | 0.742765 |
Target: 5'- uCGagGGCGuGCGCGCGCUGCUGa-- -3' miRNA: 3'- cGCagUCGUcCGUGUGCGACGACcuc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 133916 | 0.68 | 0.73696 |
Target: 5'- cGCGUCgccgccgcccuguccAGguGGUAgCACGCggGCUGGGc -3' miRNA: 3'- -CGCAG---------------UCguCCGU-GUGCGa-CGACCUc -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 84329 | 0.68 | 0.733071 |
Target: 5'- aCGUCAGCuacGGC-CG-GCUGCaGGAGg -3' miRNA: 3'- cGCAGUCGu--CCGuGUgCGACGaCCUC- -5' |
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7652 | 5' | -57.7 | NC_001973.1 | + | 60416 | 0.68 | 0.733071 |
Target: 5'- uUG-CAGCGGGCGCGCuC-GCUGGAu -3' miRNA: 3'- cGCaGUCGUCCGUGUGcGaCGACCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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