Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8063 | 5' | -57.5 | NC_001973.1 | + | 4374 | 0.66 | 0.859975 |
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Target: 5'- -gGCGGUCugGcGCU-CCGcGGAGGUCa -3' miRNA: 3'- uaUGCUAGugC-CGGcGGC-UCUCCAGc -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 143482 | 0.66 | 0.867404 |
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Target: 5'- aGUAcCGGUcCGCGGCguCGUCGAG-GGUCa -3' miRNA: 3'- -UAU-GCUA-GUGCCG--GCGGCUCuCCAGc -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 104522 | 0.66 | 0.867404 |
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Target: 5'- -aGCGA-CA-GGUCGCUGAcGAGGUUGa -3' miRNA: 3'- uaUGCUaGUgCCGGCGGCU-CUCCAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 8860 | 0.66 | 0.874629 |
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Target: 5'- -gGCGA-CACgGGCUGuUCGAGAGGUg- -3' miRNA: 3'- uaUGCUaGUG-CCGGC-GGCUCUCCAgc -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 113287 | 0.66 | 0.874629 |
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Target: 5'- -cACGAUCACGuugacGCCGCCGAacGGcuuGUCGu -3' miRNA: 3'- uaUGCUAGUGC-----CGGCGGCU--CUc--CAGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 35434 | 0.66 | 0.874629 |
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Target: 5'- -cGCGcaacgCGCGGUCGUCGAcGAGG-CGg -3' miRNA: 3'- uaUGCua---GUGCCGGCGGCU-CUCCaGC- -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 145591 | 0.66 | 0.881645 |
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Target: 5'- -cACGAgCGCGGCCGUgGAccuGAGcGUCc -3' miRNA: 3'- uaUGCUaGUGCCGGCGgCU---CUC-CAGc -5' |
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| 8063 | 5' | -57.5 | NC_001973.1 | + | 48081 | 0.66 | 0.884391 |
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Target: 5'- -cGCGuUCACGcugcgguacgugcucGCCGCCGAGcagccGGUCa -3' miRNA: 3'- uaUGCuAGUGC---------------CGGCGGCUCu----CCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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