Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 8073 | 3' | -61.8 | NC_001978.2 | + | 35159 | 0.72 | 0.129833 |
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Target: 5'- cCCGGCuucugaGUUCGCCGucaGCGcCCCGGUAc -3' miRNA: 3'- -GGCCG------CAGGCGGCug-UGCuGGGCCAU- -5' |
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| 8073 | 3' | -61.8 | NC_001978.2 | + | 35466 | 0.74 | 0.096312 |
|
Target: 5'- aCUGGCGUCCGCagcaaCGGCGuuCGGCCUGGg- -3' miRNA: 3'- -GGCCGCAGGCG-----GCUGU--GCUGGGCCau -5' |
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| 8073 | 3' | -61.8 | NC_001978.2 | + | 18107 | 0.77 | 0.058487 |
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Target: 5'- uCCGGCGccgaCGCUGACGguUGGCCCGGUAa -3' miRNA: 3'- -GGCCGCag--GCGGCUGU--GCUGGGCCAU- -5' |
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| 8073 | 3' | -61.8 | NC_001978.2 | + | 19421 | 0.78 | 0.048058 |
|
Target: 5'- gUCGGCGUCCGUCGGgACGuagcgcuccgauGCCCGGUu -3' miRNA: 3'- -GGCCGCAGGCGGCUgUGC------------UGGGCCAu -5' |
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| 8073 | 3' | -61.8 | NC_001978.2 | + | 1812 | 1.08 | 0.000259 |
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Target: 5'- uCCGGCGUCCGCCGACACGACCCGGUAa -3' miRNA: 3'- -GGCCGCAGGCGGCUGUGCUGGGCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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