Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8073 | 3' | -61.8 | NC_001978.2 | + | 35993 | 0.66 | 0.332474 |
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Target: 5'- cCCGGaGaCUGCCG-C-CGACCCGGg- -3' miRNA: 3'- -GGCCgCaGGCGGCuGuGCUGGGCCau -5' |
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| 8073 | 3' | -61.8 | NC_001978.2 | + | 36827 | 0.68 | 0.241363 |
|
Target: 5'- uCCGGCGUCgGCUccggaagGACguccugaACGGCCUGGg- -3' miRNA: 3'- -GGCCGCAGgCGG-------CUG-------UGCUGGGCCau -5' |
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| 8073 | 3' | -61.8 | NC_001978.2 | + | 37922 | 0.68 | 0.240759 |
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Target: 5'- aCGGCGcccCCGCCGACAaggGGCacuucgccccacguCCGGUAg -3' miRNA: 3'- gGCCGCa--GGCGGCUGUg--CUG--------------GGCCAU- -5' |
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| 8073 | 3' | -61.8 | NC_001978.2 | + | 38367 | 0.67 | 0.279247 |
|
Target: 5'- -gGGCGUagggUCGCCGACAUGACacaagggguugugaCCGGg- -3' miRNA: 3'- ggCCGCA----GGCGGCUGUGCUG--------------GGCCau -5' |
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| 8073 | 3' | -61.8 | NC_001978.2 | + | 38588 | 0.66 | 0.356412 |
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Target: 5'- gUCGGCGUUCGggaCGGCggaGCG-CCCGGa- -3' miRNA: 3'- -GGCCGCAGGCg--GCUG---UGCuGGGCCau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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