Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8083 | 3' | -55.3 | NC_001978.2 | + | 11579 | 0.89 | 0.021169 |
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Target: 5'- aAGGCGACGUCGCGUaaAGCUCGACCGa -3' miRNA: 3'- -UUCGCUGCAGCGCAgcUUGAGCUGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 8658 | 0.66 | 0.663881 |
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Target: 5'- --cCGACGUCG-G-CGGGCUCGAUCu -3' miRNA: 3'- uucGCUGCAGCgCaGCUUGAGCUGGu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 7628 | 1.06 | 0.001219 |
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Target: 5'- cAAGCGACGUCGCGUCGAACUCGACCAc -3' miRNA: 3'- -UUCGCUGCAGCGCAGCUUGAGCUGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 7575 | 0.75 | 0.200321 |
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Target: 5'- -cGCGACGUCGC-UUGAGCU-GACCGg -3' miRNA: 3'- uuCGCUGCAGCGcAGCUUGAgCUGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 4597 | 0.69 | 0.456221 |
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Target: 5'- cAGCGACGUCGCGaccaacggucgugUCGGGaggUCGAUCu -3' miRNA: 3'- uUCGCUGCAGCGC-------------AGCUUg--AGCUGGu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 4545 | 0.67 | 0.574468 |
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Target: 5'- -cGCGACGUCGC--UGAGCUacugaagcggggCGACCu -3' miRNA: 3'- uuCGCUGCAGCGcaGCUUGA------------GCUGGu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 2668 | 0.73 | 0.254786 |
|
Target: 5'- -cGCGuCGUCGCGcCGGAagaUCGACCu -3' miRNA: 3'- uuCGCuGCAGCGCaGCUUg--AGCUGGu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 1030 | 0.66 | 0.634775 |
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Target: 5'- cAAGCGGCGUCGgcaacggccaUGUCGAGCguguaguaacccggCGugCGg -3' miRNA: 3'- -UUCGCUGCAGC----------GCAGCUUGa-------------GCugGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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