Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8083 | 3' | -55.3 | NC_001978.2 | + | 33879 | 0.69 | 0.477679 |
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Target: 5'- cGAGCGACGUCaCGUCGAcaaACucagguUCGACg- -3' miRNA: 3'- -UUCGCUGCAGcGCAGCU---UG------AGCUGgu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 27670 | 0.69 | 0.477679 |
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Target: 5'- -cGCGaACGUCGUGgUGAuACUCGAUCAc -3' miRNA: 3'- uuCGC-UGCAGCGCaGCU-UGAGCUGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 31947 | 0.68 | 0.498568 |
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Target: 5'- aGAGCGACaGUUGU-UCGGGCUCGAauCCGu -3' miRNA: 3'- -UUCGCUG-CAGCGcAGCUUGAGCU--GGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 22407 | 0.68 | 0.509164 |
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Target: 5'- aGGGCGACGUCGggauaCGUCGccUUCGACg- -3' miRNA: 3'- -UUCGCUGCAGC-----GCAGCuuGAGCUGgu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 32628 | 0.66 | 0.630291 |
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Target: 5'- cAGCGcCGUCGUGgcaaGAACaUUGACCu -3' miRNA: 3'- uUCGCuGCAGCGCag--CUUG-AGCUGGu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 19583 | 0.66 | 0.652701 |
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Target: 5'- cGGCGcCGgucagcucCGUGUCGAACgucCGGCCGu -3' miRNA: 3'- uUCGCuGCa-------GCGCAGCUUGa--GCUGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 17859 | 0.66 | 0.663881 |
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Target: 5'- -cGCGGCauaguuggucguGUCgGCGUCGGACcaauUCGGCCc -3' miRNA: 3'- uuCGCUG------------CAG-CGCAGCUUG----AGCUGGu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 8658 | 0.66 | 0.663881 |
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Target: 5'- --cCGACGUCG-G-CGGGCUCGAUCu -3' miRNA: 3'- uucGCUGCAGCgCaGCUUGAGCUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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