Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8083 | 3' | -55.3 | NC_001978.2 | + | 37873 | 0.68 | 0.530631 |
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Target: 5'- gGGGCGccgucccguacaACGUCGCGcgUGAGCgggUCGGCCGg -3' miRNA: 3'- -UUCGC------------UGCAGCGCa-GCUUG---AGCUGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 23185 | 0.67 | 0.53605 |
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Target: 5'- uGAGCGGggcaaggucguuccCGUCGCGcUCGAcaaguggcguauCUCGACCGg -3' miRNA: 3'- -UUCGCU--------------GCAGCGC-AGCUu-----------GAGCUGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 23642 | 0.67 | 0.545852 |
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Target: 5'- gAAGaCGACGUugagCGCGUCGGcaaggguggcgcuguGCUCG-CCAa -3' miRNA: 3'- -UUC-GCUGCA----GCGCAGCU---------------UGAGCuGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 4545 | 0.67 | 0.574468 |
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Target: 5'- -cGCGACGUCGC--UGAGCUacugaagcggggCGACCu -3' miRNA: 3'- uuCGCUGCAGCGcaGCUUGA------------GCUGGu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 1030 | 0.66 | 0.634775 |
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Target: 5'- cAAGCGGCGUCGgcaacggccaUGUCGAGCguguaguaacccggCGugCGg -3' miRNA: 3'- -UUCGCUGCAGC----------GCAGCUUGa-------------GCugGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 22738 | 0.66 | 0.640379 |
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Target: 5'- cGGCGucguguuCGUCGCugccgucGUCGGGCUCGcCCu -3' miRNA: 3'- uUCGCu------GCAGCG-------CAGCUUGAGCuGGu -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 20382 | 0.66 | 0.645982 |
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Target: 5'- gAAGCGGCGaaggccgaagucgagUCGCGcCGG--UCGGCCGa -3' miRNA: 3'- -UUCGCUGC---------------AGCGCaGCUugAGCUGGU- -5' |
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| 8083 | 3' | -55.3 | NC_001978.2 | + | 17588 | 0.76 | 0.160696 |
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Target: 5'- cGGCGACGUUGgcgaCGUUGGcgGCUCGACCAc -3' miRNA: 3'- uUCGCUGCAGC----GCAGCU--UGAGCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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