Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8084 | 5' | -61.6 | NC_001978.2 | + | 17641 | 0.66 | 0.328871 |
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Target: 5'- cGUCcggaGCCGUCGCCCGguacaaGGUgaccaucaacggucuUGAGUACg -3' miRNA: 3'- -CAGug--CGGCAGCGGGC------CCA---------------GCUCGUG- -5' |
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| 8084 | 5' | -61.6 | NC_001978.2 | + | 39562 | 0.66 | 0.324977 |
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Target: 5'- -cCGCuGCCGUCGCCgGaagguGGUCGAcccguccGCACc -3' miRNA: 3'- caGUG-CGGCAGCGGgC-----CCAGCU-------CGUG- -5' |
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| 8084 | 5' | -61.6 | NC_001978.2 | + | 3852 | 0.66 | 0.325753 |
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Target: 5'- uUCA-GUCuUCGCCCGuGGcUUGAGCGCa -3' miRNA: 3'- cAGUgCGGcAGCGGGC-CC-AGCUCGUG- -5' |
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| 8084 | 5' | -61.6 | NC_001978.2 | + | 200 | 0.67 | 0.30307 |
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Target: 5'- aGUC-CGCUGcC-CCCgucguguacgaGGGUCGAGCGCc -3' miRNA: 3'- -CAGuGCGGCaGcGGG-----------CCCAGCUCGUG- -5' |
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| 8084 | 5' | -61.6 | NC_001978.2 | + | 7930 | 0.67 | 0.302335 |
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Target: 5'- cUCACGUaCGUCgaGCCCGgaacgccGGUCGAGC-Cg -3' miRNA: 3'- cAGUGCG-GCAG--CGGGC-------CCAGCUCGuG- -5' |
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| 8084 | 5' | -61.6 | NC_001978.2 | + | 24769 | 0.67 | 0.295783 |
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Target: 5'- aGUCagaaGCGUCGggCGacggcauaCgGGGUCGAGCACg -3' miRNA: 3'- -CAG----UGCGGCa-GCg-------GgCCCAGCUCGUG- -5' |
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| 8084 | 5' | -61.6 | NC_001978.2 | + | 10102 | 0.67 | 0.295783 |
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Target: 5'- aUCaACGCCGguaaggcaguggUCGCCUGGGUgaCGucaAGCGCg -3' miRNA: 3'- cAG-UGCGGC------------AGCGGGCCCA--GC---UCGUG- -5' |
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| 8084 | 5' | -61.6 | NC_001978.2 | + | 30371 | 0.67 | 0.288634 |
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Target: 5'- -aCGCGCCGaagcCGCCCGGcUCGcguGGCAg -3' miRNA: 3'- caGUGCGGCa---GCGGGCCcAGC---UCGUg -5' |
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| 8084 | 5' | -61.6 | NC_001978.2 | + | 13253 | 0.72 | 0.128579 |
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Target: 5'- cUCACGCUGaacgCGUCCGGGU--GGCACa -3' miRNA: 3'- cAGUGCGGCa---GCGGGCCCAgcUCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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