Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8086 | 3' | -58 | NC_001978.2 | + | 9875 | 0.67 | 0.4095 |
|
Target: 5'- --cGCCGCGC-CGUC-GACCuugcccaGCGUCu -3' miRNA: 3'- guaCGGCGCGuGCAGgCUGG-------UGCAGu -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 8782 | 1.08 | 0.000458 |
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Target: 5'- cCAUGCCGCGCACGUCCGACCACGUCAa -3' miRNA: 3'- -GUACGGCGCGUGCAGGCUGGUGCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 8680 | 0.67 | 0.419802 |
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Target: 5'- -uUGCgUGCGCACGUCUGGUCA-GUCAc -3' miRNA: 3'- guACG-GCGCGUGCAGGCUGGUgCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 6805 | 0.66 | 0.509509 |
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Target: 5'- -cUGCCGUGaACG-CCGACCuugUGUCGg -3' miRNA: 3'- guACGGCGCgUGCaGGCUGGu--GCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 5656 | 0.69 | 0.323435 |
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Target: 5'- gAUGCCGCGCGgcugaccCGUgCCGguACC-CGUCAg -3' miRNA: 3'- gUACGGCGCGU-------GCA-GGC--UGGuGCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 2046 | 0.67 | 0.419802 |
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Target: 5'- aCGUGUgGCGCGCGUgUaACC-CGUCGu -3' miRNA: 3'- -GUACGgCGCGUGCAgGcUGGuGCAGU- -5' |
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| 8086 | 3' | -58 | NC_001978.2 | + | 1364 | 0.66 | 0.478619 |
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Target: 5'- uGUGCCGCGCAaucacgggguCGauUCCGgcccaguccGCCACGgUCAc -3' miRNA: 3'- gUACGGCGCGU----------GC--AGGC---------UGGUGC-AGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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