Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8087 | 3' | -58.4 | NC_001978.2 | + | 30518 | 0.66 | 0.47036 |
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Target: 5'- uGCUUAGCgugUCGGCaagGGAaccGUCGgCGUCCg -3' miRNA: 3'- -CGAAUCGg--AGUCGg--CCU---CAGC-GCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 26051 | 0.66 | 0.480464 |
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Target: 5'- gGCgucguGCagUCGGCUGGggaacuugcccgGGUCGCGCUCa -3' miRNA: 3'- -CGaau--CGg-AGUCGGCC------------UCAGCGCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 35561 | 0.66 | 0.480464 |
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Target: 5'- ----cGCCggaugUCgAGCCGGAGaCGgCGCCCc -3' miRNA: 3'- cgaauCGG-----AG-UCGGCCUCaGC-GCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 38171 | 0.66 | 0.490674 |
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Target: 5'- gGCgcGGCUUCcccguGCCGGAGcaauCGCCCu -3' miRNA: 3'- -CGaaUCGGAGu----CGGCCUCagc-GCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 26189 | 0.66 | 0.490674 |
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Target: 5'- cGCUugUGGCauggacugaCGGCCGGAucaaGUaCGCGUCCg -3' miRNA: 3'- -CGA--AUCGga-------GUCGGCCU----CA-GCGCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 27099 | 0.66 | 0.490674 |
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Target: 5'- ---aAGCUUCGGgCGGAGUCG-GCg- -3' miRNA: 3'- cgaaUCGGAGUCgGCCUCAGCgCGgg -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 32181 | 0.66 | 0.490674 |
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Target: 5'- aGCUgacgcGCCUgAGCCGGGGUCaucacggcacgGuCGgCCg -3' miRNA: 3'- -CGAau---CGGAgUCGGCCUCAG-----------C-GCgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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