Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8087 | 3' | -58.4 | NC_001978.2 | + | 18163 | 0.67 | 0.421559 |
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Target: 5'- ---cAGCgUCGgcGCCGGAGUaacCGCGCUUg -3' miRNA: 3'- cgaaUCGgAGU--CGGCCUCA---GCGCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 27144 | 0.67 | 0.412168 |
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Target: 5'- aGCUUccGGUCaaGGUCGuAGUCGCGCUCu -3' miRNA: 3'- -CGAA--UCGGagUCGGCcUCAGCGCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 23231 | 0.67 | 0.393782 |
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Target: 5'- cGCUcauucGUCUCgcuguAGCCGGAGUgGaaaCGCCCa -3' miRNA: 3'- -CGAau---CGGAG-----UCGGCCUCAgC---GCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 7550 | 0.67 | 0.384791 |
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Target: 5'- ----cGCCUCAGCgGGAuagGUCG-GCUCg -3' miRNA: 3'- cgaauCGGAGUCGgCCU---CAGCgCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 11390 | 0.68 | 0.36722 |
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Target: 5'- gGCUUccgugaaCUCAGCgCGG--UCGCGCCCg -3' miRNA: 3'- -CGAAucg----GAGUCG-GCCucAGCGCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 4968 | 0.68 | 0.358644 |
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Target: 5'- cGCgc-GCCUCaaAGuuGGcgcucagaguGGUCGCGUCCa -3' miRNA: 3'- -CGaauCGGAG--UCggCC----------UCAGCGCGGG- -5' |
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| 8087 | 3' | -58.4 | NC_001978.2 | + | 32181 | 0.66 | 0.490674 |
|
Target: 5'- aGCUgacgcGCCUgAGCCGGGGUCaucacggcacgGuCGgCCg -3' miRNA: 3'- -CGAau---CGGAgUCGGCCUCAG-----------C-GCgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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