Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8113 | 3' | -54.7 | NC_001978.2 | + | 30506 | 0.66 | 0.674868 |
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Target: 5'- cGGcAAGGGAaccGUCGGCGuCCgCGUUcucaGCCg -3' miRNA: 3'- -UCaUUCCCU---CAGCCGUuGG-GCGA----UGG- -5' |
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| 8113 | 3' | -54.7 | NC_001978.2 | + | 31220 | 0.83 | 0.057783 |
|
Target: 5'- cAGUAAGGaAGUCGGCAACCCGggGCg -3' miRNA: 3'- -UCAUUCCcUCAGCCGUUGGGCgaUGg -5' |
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| 8113 | 3' | -54.7 | NC_001978.2 | + | 35528 | 0.76 | 0.178266 |
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Target: 5'- -----cGGAGaCGGCAuACCCGCUGCCg -3' miRNA: 3'- ucauucCCUCaGCCGU-UGGGCGAUGG- -5' |
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| 8113 | 3' | -54.7 | NC_001978.2 | + | 36248 | 0.97 | 0.005798 |
|
Target: 5'- cAGUAAGGGAGUCGGCAACCCGggACUa -3' miRNA: 3'- -UCAUUCCCUCAGCCGUUGGGCgaUGG- -5' |
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| 8113 | 3' | -54.7 | NC_001978.2 | + | 40350 | 0.68 | 0.573879 |
|
Target: 5'- --cGGGGGAc-CGGCcgacccuuCCCGCUGCCc -3' miRNA: 3'- ucaUUCCCUcaGCCGuu------GGGCGAUGG- -5' |
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| 8113 | 3' | -54.7 | NC_001978.2 | + | 40697 | 0.71 | 0.388435 |
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Target: 5'- cGUAaucAGGGGGUCGGCggUUCGaauCCg -3' miRNA: 3'- uCAU---UCCCUCAGCCGuuGGGCgauGG- -5' |
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| 8113 | 3' | -54.7 | NC_001978.2 | + | 41223 | 0.66 | 0.674868 |
|
Target: 5'- cGUAAGGcAGUgGGCGcgcACUgUGCUACCu -3' miRNA: 3'- uCAUUCCcUCAgCCGU---UGG-GCGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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