Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8164 | 3' | -59.6 | NC_001978.2 | + | 18930 | 0.66 | 0.39891 |
Target: 5'- aGCaugGUUCgG-GUGaCUCGACCCGGcacGCu -3' miRNA: 3'- aCGg--CAAGgCaCAC-GAGCUGGGCC---CG- -5' |
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8164 | 3' | -59.6 | NC_001978.2 | + | 39188 | 0.66 | 0.39891 |
Target: 5'- aGCC---CCGUGaUGCU--GCCCGGGUg -3' miRNA: 3'- aCGGcaaGGCAC-ACGAgcUGGGCCCG- -5' |
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8164 | 3' | -59.6 | NC_001978.2 | + | 16016 | 0.66 | 0.433143 |
Target: 5'- gGCCGgUCCGgaggGUccccaggguccgaaGggUGACCCGGGUa -3' miRNA: 3'- aCGGCaAGGCa---CA--------------CgaGCUGGGCCCG- -5' |
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8164 | 3' | -59.6 | NC_001978.2 | + | 22447 | 0.66 | 0.445571 |
Target: 5'- cUGCCGUUCUGcacGUGaCUgacgaGACgUGGGCu -3' miRNA: 3'- -ACGGCAAGGCa--CAC-GAg----CUGgGCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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