Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8207 | 5' | -55.6 | NC_001978.2 | + | 36072 | 0.66 | 0.658874 |
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Target: 5'- uGCUACacgGGCCAcucaccCGCCGACACcUGGg -3' miRNA: 3'- gCGAUGcagCUGGU------GCGGCUGUG-ACU- -5' |
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| 8207 | 5' | -55.6 | NC_001978.2 | + | 37382 | 0.68 | 0.536798 |
|
Target: 5'- aGCaacgGCGcCGACCaucgauaGCGCCGcugagGCACUGAc -3' miRNA: 3'- gCGa---UGCaGCUGG-------UGCGGC-----UGUGACU- -5' |
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| 8207 | 5' | -55.6 | NC_001978.2 | + | 39787 | 0.67 | 0.559523 |
|
Target: 5'- uGUUGCG--GAgCGCGCCGACGCccUGAa -3' miRNA: 3'- gCGAUGCagCUgGUGCGGCUGUG--ACU- -5' |
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| 8207 | 5' | -55.6 | NC_001978.2 | + | 39965 | 0.69 | 0.468804 |
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Target: 5'- aGCUuccCcUUGACCaaugguuccccgaagACGCCGACGCUGAc -3' miRNA: 3'- gCGAu--GcAGCUGG---------------UGCGGCUGUGACU- -5' |
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| 8207 | 5' | -55.6 | NC_001978.2 | + | 40260 | 0.69 | 0.485156 |
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Target: 5'- cCGCUACGUCuuCCGUGCCGuC-CUGGg -3' miRNA: 3'- -GCGAUGCAGcuGGUGCGGCuGuGACU- -5' |
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| 8207 | 5' | -55.6 | NC_001978.2 | + | 41278 | 0.74 | 0.241883 |
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Target: 5'- aGCUcgACGUCGACCACaucaaGCCGcuuGCAUUGGg -3' miRNA: 3'- gCGA--UGCAGCUGGUG-----CGGC---UGUGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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