miRNA: 3'- -UGCGACUG-GGC-UGGCcGGGaUUCaGC- -5'
miRNA: 3'- -UGCGacUGGGCUGGCCGGGAu-----UCaGC- -5'
miRNA: 3'- ugCGA-CUGGG------CUGGC-CGGGaUUCAGC- -5'
miRNA: 3'- ugCGAcuGGgCUGGCCGGGauuCAGC- -5'
miRNA: 3'- -UGCGaCUGGGCU-----GGCCGGGa--UUCAGc -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8223 | 3' | -59 | NC_001978.2 | + | 39161 | 0.66 | 0.448902 |
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Target: 5'- gACGCUGACgCCGuGCCGaCCCgGGG-CGa -3' miRNA: 3'- -UGCGACUG-GGC-UGGCcGGGaUUCaGC- -5' |
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| 8223 | 3' | -59 | NC_001978.2 | + | 17183 | 0.66 | 0.458696 |
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Target: 5'- aAUGUccGCCCGACCGGCCUUcgugauAGcCGa -3' miRNA: 3'- -UGCGacUGGGCUGGCCGGGAu-----UCaGC- -5' |
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| 8223 | 3' | -59 | NC_001978.2 | + | 26019 | 0.66 | 0.458696 |
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Target: 5'- -gGCUcGACCCguccgcGACCGuGCCC-AGGUUGa -3' miRNA: 3'- ugCGA-CUGGG------CUGGC-CGGGaUUCAGC- -5' |
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| 8223 | 3' | -59 | NC_001978.2 | + | 22350 | 0.66 | 0.458696 |
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Target: 5'- -gGCUucCCgGACCGGUCCggcGUCGg -3' miRNA: 3'- ugCGAcuGGgCUGGCCGGGauuCAGC- -5' |
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| 8223 | 3' | -59 | NC_001978.2 | + | 15955 | 0.66 | 0.468603 |
|
Target: 5'- gGCGCcGACUCGAcguucCCGGUCCccaGGGUCc -3' miRNA: 3'- -UGCGaCUGGGCU-----GGCCGGGa--UUCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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