Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8481 | 5' | -54 | NC_002169.1 | + | 43316 | 0.66 | 0.961458 |
Target: 5'- cGUCGUUGAccaucGACACGguGCUCUUGAa-- -3' miRNA: 3'- aCGGCAGCU-----CUGUGC--UGAGAACUggg -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 114103 | 0.66 | 0.959301 |
Target: 5'- aGCUGUCGAcGAgGCGACUCaauucgauaaaacGAgCCg -3' miRNA: 3'- aCGGCAGCU-CUgUGCUGAGaa-----------CUgGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 96508 | 0.66 | 0.957819 |
Target: 5'- aGCUGUUGAagaagacccgcGACGCG-CUCUUG-CCg -3' miRNA: 3'- aCGGCAGCU-----------CUGUGCuGAGAACuGGg -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 100953 | 0.66 | 0.957819 |
Target: 5'- -uUCGUCGuAGACA-GACUUguaguuccuUUGGCCCa -3' miRNA: 3'- acGGCAGC-UCUGUgCUGAG---------AACUGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 92780 | 0.66 | 0.956683 |
Target: 5'- aUGUCcUCGAGcgucuugagaccgcACGCGACgcugUGGCCCa -3' miRNA: 3'- -ACGGcAGCUC--------------UGUGCUGaga-ACUGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 128801 | 0.66 | 0.953952 |
Target: 5'- gUGCCGUCGAGcCAUG-Cg--UGACgCu -3' miRNA: 3'- -ACGGCAGCUCuGUGCuGagaACUGgG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 132954 | 0.66 | 0.953952 |
Target: 5'- aUGCCGUCuuGAagaGAUUCUUGACa- -3' miRNA: 3'- -ACGGCAGcuCUgugCUGAGAACUGgg -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 90814 | 0.66 | 0.949852 |
Target: 5'- cUGuuGUCGAGGCAUuuuugaGcACUCUUGaAUCUa -3' miRNA: 3'- -ACggCAGCUCUGUG------C-UGAGAAC-UGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 36933 | 0.66 | 0.945516 |
Target: 5'- aGCCGaCGGGGCuuuCGGCgag-GAUCCa -3' miRNA: 3'- aCGGCaGCUCUGu--GCUGagaaCUGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 28845 | 0.66 | 0.945516 |
Target: 5'- uUGCaacaGUCGA-ACGCGACUU-UGGCCa -3' miRNA: 3'- -ACGg---CAGCUcUGUGCUGAGaACUGGg -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 54109 | 0.67 | 0.940942 |
Target: 5'- gUGCCGUCccgcuGGCcaagaGACUUUUGGCCa -3' miRNA: 3'- -ACGGCAGcu---CUGug---CUGAGAACUGGg -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 85629 | 0.67 | 0.931067 |
Target: 5'- cGCCGU-GAGAaauuggaaaacgUACGACU--UGGCCCc -3' miRNA: 3'- aCGGCAgCUCU------------GUGCUGAgaACUGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 34520 | 0.67 | 0.925765 |
Target: 5'- cGCCGUCGccGAUAUuGACUCUaaUGACg- -3' miRNA: 3'- aCGGCAGCu-CUGUG-CUGAGA--ACUGgg -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 115305 | 0.67 | 0.925765 |
Target: 5'- cGCUGUCGAGAgCAgGACacaccGAUCCg -3' miRNA: 3'- aCGGCAGCUCU-GUgCUGagaa-CUGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 45636 | 0.68 | 0.908396 |
Target: 5'- aGCgG-CGAGACGCGugUCga-GCUCg -3' miRNA: 3'- aCGgCaGCUCUGUGCugAGaacUGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 34852 | 0.68 | 0.90778 |
Target: 5'- aGgCGUCGAG-CGCGgggauguggccguACUCgacGACCCa -3' miRNA: 3'- aCgGCAGCUCuGUGC-------------UGAGaa-CUGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 35987 | 0.68 | 0.902124 |
Target: 5'- cUGCCGUCG-GGCAU--CUCUUcGuCCCa -3' miRNA: 3'- -ACGGCAGCuCUGUGcuGAGAA-CuGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 51920 | 0.68 | 0.888869 |
Target: 5'- cGCUGaUUGAGACGCGAag--UGGCCa -3' miRNA: 3'- aCGGC-AGCUCUGUGCUgagaACUGGg -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 42945 | 0.7 | 0.791581 |
Target: 5'- aGCgUGUCGAGcgcuaGCACGGCUUgcggcaggaUGGCCCg -3' miRNA: 3'- aCG-GCAGCUC-----UGUGCUGAGa--------ACUGGG- -5' |
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8481 | 5' | -54 | NC_002169.1 | + | 26725 | 0.7 | 0.791581 |
Target: 5'- cUGUCGUCGAGuauGCACGACacgUUU-ACCCa -3' miRNA: 3'- -ACGGCAGCUC---UGUGCUGa--GAAcUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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