Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8481 | 5' | -54 | NC_002169.1 | + | 88900 | 0.71 | 0.781392 |
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Target: 5'- aGCCugggauuGUUGAGACACG-CUCgucUGugCCg -3' miRNA: 3'- aCGG-------CAGCUCUGUGCuGAGa--ACugGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 127499 | 0.71 | 0.76341 |
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Target: 5'- --aCGUCGAGACAcCGACUCccagcGAUCCc -3' miRNA: 3'- acgGCAGCUCUGU-GCUGAGaa---CUGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 30845 | 0.71 | 0.74402 |
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Target: 5'- cGCgGUCGcGACACGACaCgUUGGCCg -3' miRNA: 3'- aCGgCAGCuCUGUGCUGaG-AACUGGg -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 88659 | 0.72 | 0.734171 |
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Target: 5'- cUGUCGUCGAGAguCGCGAU--UUGugCCu -3' miRNA: 3'- -ACGGCAGCUCU--GUGCUGagAACugGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 33029 | 0.76 | 0.481235 |
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Target: 5'- cGUCGUCGAcaaacuuauCACGACUCUUGuauCCCa -3' miRNA: 3'- aCGGCAGCUcu-------GUGCUGAGAACu--GGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 118920 | 0.8 | 0.319419 |
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Target: 5'- uUGUCGUCGGGAUuuUGGCgccgCUUGACCCg -3' miRNA: 3'- -ACGGCAGCUCUGu-GCUGa---GAACUGGG- -5' |
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| 8481 | 5' | -54 | NC_002169.1 | + | 2938 | 1.13 | 0.002444 |
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Target: 5'- gUGCCGUCGAGACACGACUCUUGACCCa -3' miRNA: 3'- -ACGGCAGCUCUGUGCUGAGAACUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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