Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8482 | 5' | -53.8 | NC_002169.1 | + | 37973 | 0.67 | 0.918593 |
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Target: 5'- cGCCGGaguaGACAaacUUGACGCUGACGu -3' miRNA: 3'- aUGGCUguggCUGU---GACUGUGACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 35588 | 0.69 | 0.864504 |
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Target: 5'- aUAUCGGCACCGACA--GACACUugGAUu -3' miRNA: 3'- -AUGGCUGUGGCUGUgaCUGUGA--CUGc -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 34520 | 0.74 | 0.563612 |
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Target: 5'- cGCCGuCGCCGAUAUUGACucuaaUGACGu -3' miRNA: 3'- aUGGCuGUGGCUGUGACUGug---ACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 26353 | 0.66 | 0.944464 |
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Target: 5'- cGCCGGCGacgUCGuuGCGCUGcCAuCUGACGa -3' miRNA: 3'- aUGGCUGU---GGC--UGUGACuGU-GACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 15834 | 0.7 | 0.805762 |
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Target: 5'- gACCGcCGCCGACGCUGcCGCaauGCGu -3' miRNA: 3'- aUGGCuGUGGCUGUGACuGUGac-UGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 14362 | 0.67 | 0.918593 |
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Target: 5'- aAUCGAUACCGAUGCUGGaaaacCACgaaGACa -3' miRNA: 3'- aUGGCUGUGGCUGUGACU-----GUGa--CUGc -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 14021 | 0.69 | 0.848704 |
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Target: 5'- aUGCCcACGCCGGCGCgcaACAC-GACGg -3' miRNA: 3'- -AUGGcUGUGGCUGUGac-UGUGaCUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 4598 | 1.08 | 0.00486 |
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Target: 5'- aUACCGACACCGACACUGACACUGACGa -3' miRNA: 3'- -AUGGCUGUGGCUGUGACUGUGACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 1536 | 0.67 | 0.918593 |
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Target: 5'- cUAUUGACGuuGACAUuggUGGCGgUGGCGg -3' miRNA: 3'- -AUGGCUGUggCUGUG---ACUGUgACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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