Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8482 | 5' | -53.8 | NC_002169.1 | + | 75461 | 0.71 | 0.739048 |
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Target: 5'- cACCGACGaagaCGAUAgaGACGgUGACGa -3' miRNA: 3'- aUGGCUGUg---GCUGUgaCUGUgACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 120407 | 0.7 | 0.796648 |
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Target: 5'- aAUCGAUGuuGAUGUUGGCGCUGACGa -3' miRNA: 3'- aUGGCUGUggCUGUGACUGUGACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 15834 | 0.7 | 0.805762 |
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Target: 5'- gACCGcCGCCGACGCUGcCGCaauGCGu -3' miRNA: 3'- aUGGCuGUGGCUGUGACuGUGac-UGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 99866 | 0.68 | 0.886557 |
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Target: 5'- -uCCGACGaugaCGACAUUGACGaguaUGAUGa -3' miRNA: 3'- auGGCUGUg---GCUGUGACUGUg---ACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 56592 | 0.68 | 0.900098 |
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Target: 5'- cAUCGACGCCGAUcucguaaacGCgGGCGCcGGCGu -3' miRNA: 3'- aUGGCUGUGGCUG---------UGaCUGUGaCUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 1536 | 0.67 | 0.918593 |
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Target: 5'- cUAUUGACGuuGACAUuggUGGCGgUGGCGg -3' miRNA: 3'- -AUGGCUGUggCUGUG---ACUGUgACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 61508 | 0.67 | 0.918593 |
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Target: 5'- cACCGuCGCCGAUcuaauACUcaacGACACgGACGa -3' miRNA: 3'- aUGGCuGUGGCUG-----UGA----CUGUGaCUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 82550 | 0.67 | 0.93486 |
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Target: 5'- gGCCGACAUgucaaaguUGACuCUGACGCgcaugcgGGCGu -3' miRNA: 3'- aUGGCUGUG--------GCUGuGACUGUGa------CUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 99394 | 0.84 | 0.168271 |
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Target: 5'- cACCacaGAUACCGACACUGACACUGAa- -3' miRNA: 3'- aUGG---CUGUGGCUGUGACUGUGACUgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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