Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8482 | 5' | -53.8 | NC_002169.1 | + | 15834 | 0.7 | 0.805762 |
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Target: 5'- gACCGcCGCCGACGCUGcCGCaauGCGu -3' miRNA: 3'- aUGGCuGUGGCUGUGACuGUGac-UGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 120407 | 0.7 | 0.796648 |
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Target: 5'- aAUCGAUGuuGAUGUUGGCGCUGACGa -3' miRNA: 3'- aUGGCUGUggCUGUGACUGUGACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 75461 | 0.71 | 0.739048 |
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Target: 5'- cACCGACGaagaCGAUAgaGACGgUGACGa -3' miRNA: 3'- aUGGCUGUg---GCUGUgaCUGUgACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 61843 | 0.71 | 0.729051 |
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Target: 5'- cGCCGAUcCCGACGgacacauuaauCUGuCGCUGGCGa -3' miRNA: 3'- aUGGCUGuGGCUGU-----------GACuGUGACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 90235 | 0.72 | 0.688291 |
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Target: 5'- cGCCGugGuCUGACgACUaucGGCGCUGACGa -3' miRNA: 3'- aUGGCugU-GGCUG-UGA---CUGUGACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 68647 | 0.72 | 0.688291 |
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Target: 5'- cGCCGGUACCGGCAUUccCACUGACu -3' miRNA: 3'- aUGGCUGUGGCUGUGAcuGUGACUGc -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 34520 | 0.74 | 0.563612 |
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Target: 5'- cGCCGuCGCCGAUAUUGACucuaaUGACGu -3' miRNA: 3'- aUGGCuGUGGCUGUGACUGug---ACUGC- -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 99394 | 0.84 | 0.168271 |
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Target: 5'- cACCacaGAUACCGACACUGACACUGAa- -3' miRNA: 3'- aUGG---CUGUGGCUGUGACUGUGACUgc -5' |
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| 8482 | 5' | -53.8 | NC_002169.1 | + | 4598 | 1.08 | 0.00486 |
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Target: 5'- aUACCGACACCGACACUGACACUGACGa -3' miRNA: 3'- -AUGGCUGUGGCUGUGACUGUGACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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