miRNA: 3'- guuaaUGGgu-UUUgUUCGU-ACGCGGC- -5'
miRNA: 3'- guuaaUGGguUUugUUCGUACGCGGc -5'
miRNA: 3'- guuaAUGGGUuu----UGUUCGu----ACgCGGC- -5'
miRNA: 3'- guuaAUGGGUuu----UGUUCGu----ACgCGGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8638 | 3' | -46.9 | NC_002229.2 | + | 67419 | 0.66 | 0.999912 |
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Target: 5'- ----cGCCauuAAAgAAGCAgUGCGCCGc -3' miRNA: 3'- guuaaUGGgu-UUUgUUCGU-ACGCGGC- -5' |
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| 8638 | 3' | -46.9 | NC_002229.2 | + | 55033 | 0.66 | 0.999912 |
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Target: 5'- ----gGCUguGAugGAGUAUGCGUCa -3' miRNA: 3'- guuaaUGGguUUugUUCGUACGCGGc -5' |
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| 8638 | 3' | -46.9 | NC_002229.2 | + | 169379 | 0.66 | 0.999933 |
|
Target: 5'- ---gUGCCCAuuugccGCAGGCuguacUGgGCCGg -3' miRNA: 3'- guuaAUGGGUuu----UGUUCGu----ACgCGGC- -5' |
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| 8638 | 3' | -46.9 | NC_002229.2 | + | 143235 | 0.66 | 0.999933 |
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Target: 5'- ---gUGCCCAuuugccGCAGGCuguacUGgGCCGg -3' miRNA: 3'- guuaAUGGGUuu----UGUUCGu----ACgCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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