Results 21 - 40 of 54 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8650 | 5' | -46.1 | NC_002229.2 | + | 4816 | 0.67 | 0.999944 |
Target: 5'- aCCCCCCCuccucccgaugcGGAGGAGCUuUGc -3' miRNA: 3'- -GGGGGGGuuuuuuu-----UUUCCUUGGcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 4939 | 0.67 | 0.999944 |
Target: 5'- aCCCCCCCuccucccgauacGGAGGAACUuUGc -3' miRNA: 3'- -GGGGGGGuuuuuuu-----UUUCCUUGGcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 143491 | 0.67 | 0.999927 |
Target: 5'- --aCCCCAAGAcccGAAAGGGACCcUGc -3' miRNA: 3'- gggGGGGUUUUuu-UUUUCCUUGGcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 147399 | 0.68 | 0.99977 |
Target: 5'- uCCCCCCCGAGcccuccccAGGcuCCGUc -3' miRNA: 3'- -GGGGGGGUUUuuuuuu--UCCuuGGCAc -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 150479 | 0.68 | 0.999902 |
Target: 5'- uUCCCCCCGccuauAGAGAGGuuaacccAGGAgacacGCUGUGg -3' miRNA: 3'- -GGGGGGGUu----UUUUUUU-------UCCU-----UGGCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 121714 | 0.68 | 0.999875 |
Target: 5'- uCCgaCCCCAGAGAGGGA--GAGCCGg- -3' miRNA: 3'- -GGg-GGGGUUUUUUUUUucCUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 122208 | 0.68 | 0.999875 |
Target: 5'- uCCgaCCCCAGAGAGGGA--GAGCCGg- -3' miRNA: 3'- -GGg-GGGGUUUUUUUUUucCUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 162135 | 0.68 | 0.999902 |
Target: 5'- uUCCCCCCGccuauAGAGAGGuuaacccAGGAgacacGCUGUGg -3' miRNA: 3'- -GGGGGGGUu----UUUUUUU-------UCCU-----UGGCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 17 | 0.68 | 0.999791 |
Target: 5'- aCCCCggCCCAcaGGAAGGGgcGGGGCaCGUGc -3' miRNA: 3'- -GGGG--GGGU--UUUUUUUuuCCUUG-GCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 165215 | 0.68 | 0.99977 |
Target: 5'- uCCCCCCCGAGcccuccccAGGcuCCGUc -3' miRNA: 3'- -GGGGGGGUUUuuuuuu--UCCuuGGCAc -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 138694 | 0.68 | 0.999791 |
Target: 5'- aCCCCggCCCAcaGGAAGGGgcGGGGCaCGUGc -3' miRNA: 3'- -GGGG--GGGU--UUUUUUUuuCCUUG-GCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 32160 | 0.69 | 0.999335 |
Target: 5'- gCCCCCCAGccuGGGAuAGGugagacCCGUGu -3' miRNA: 3'- gGGGGGGUUuu-UUUUuUCCuu----GGCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 165795 | 0.7 | 0.998323 |
Target: 5'- cUCCCCCCAGAAAuguccccggauaaucGAacugccGAGGGAACCc-- -3' miRNA: 3'- -GGGGGGGUUUUU---------------UU------UUUCCUUGGcac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 147787 | 0.7 | 0.998503 |
Target: 5'- -gUCCCCGGAuuc-GGGGGAACCGUc -3' miRNA: 3'- ggGGGGGUUUuuuuUUUCCUUGGCAc -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 142314 | 0.7 | 0.998991 |
Target: 5'- gCCCUCCCGGGGGAGuaucuGGAGCgGg- -3' miRNA: 3'- -GGGGGGGUUUUUUUuuu--CCUUGgCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 170300 | 0.7 | 0.998991 |
Target: 5'- gCCCUCCCGGGGGAGuaucuGGAGCgGg- -3' miRNA: 3'- -GGGGGGGUUUUUUUuuu--CCUUGgCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 164827 | 0.7 | 0.998503 |
Target: 5'- -gUCCCCGGAuuc-GGGGGAACCGUc -3' miRNA: 3'- ggGGGGGUUUuuuuUUUCCUUGGCAc -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 146819 | 0.7 | 0.998323 |
Target: 5'- cUCCCCCCAGAAAuguccccggauaaucGAacugccGAGGGAACCc-- -3' miRNA: 3'- -GGGGGGGUUUUU---------------UU------UUUCCUUGGcac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 12428 | 0.71 | 0.996358 |
Target: 5'- gCUCCCCAGGGuGGAAAAGGGGCgGa- -3' miRNA: 3'- gGGGGGGUUUU-UUUUUUCCUUGgCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 98876 | 0.71 | 0.996358 |
Target: 5'- aCCCCCCUucGAAcGAGAAGGGAaacuuGCCa-- -3' miRNA: 3'- -GGGGGGG--UUUuUUUUUUCCU-----UGGcac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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