Results 21 - 40 of 54 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8650 | 5' | -46.1 | NC_002229.2 | + | 127871 | 0.67 | 0.999951 |
Target: 5'- cCCCCCCCAcaaaaaaaaacaaaaAAAAAAAacugucuuauAAGGAGCa-UGg -3' miRNA: 3'- -GGGGGGGU---------------UUUUUUU----------UUCCUUGgcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 168228 | 0.67 | 0.999978 |
Target: 5'- aCUCUUCCGAAAcgGAAAAGGGAAUCu-- -3' miRNA: 3'- -GGGGGGGUUUU--UUUUUUCCUUGGcac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 134117 | 0.66 | 0.999986 |
Target: 5'- uCUCCCCCAcauccggcuccGGAGCCGg- -3' miRNA: 3'- -GGGGGGGUuuuuuuuuu--CCUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 106174 | 0.66 | 0.999988 |
Target: 5'- gCCCCCUCugcau--AAAGGAGauCCGUa -3' miRNA: 3'- -GGGGGGGuuuuuuuUUUCCUU--GGCAc -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 131797 | 0.66 | 0.999992 |
Target: 5'- -aCCCCCGAG---GGAAGGAAaaaGUGc -3' miRNA: 3'- ggGGGGGUUUuuuUUUUCCUUgg-CAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 150422 | 0.66 | 0.999994 |
Target: 5'- ------aAAAAAAAAGAGGAACCGUGc -3' miRNA: 3'- gggggggUUUUUUUUUUCCUUGGCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 162146 | 1.11 | 0.024115 |
Target: 5'- cCCCCCCCAAAAAAAAAAGGAACCGUGc -3' miRNA: 3'- -GGGGGGGUUUUUUUUUUCCUUGGCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 121714 | 0.68 | 0.999875 |
Target: 5'- uCCgaCCCCAGAGAGGGA--GAGCCGg- -3' miRNA: 3'- -GGg-GGGGUUUUUUUUUucCUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 6914 | 0.66 | 0.999992 |
Target: 5'- -aCCCCCGAG---GGAAGGAAaaaGUGc -3' miRNA: 3'- ggGGGGGUUUuuuUUUUCCUUgg-CAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 150901 | 0.66 | 0.99999 |
Target: 5'- cCCCCCCCuuuuuuuuucGAGuuucgcuuGGACCGUGu -3' miRNA: 3'- -GGGGGGGuuuuuuuu--UUC--------CUUGGCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 107011 | 0.66 | 0.999988 |
Target: 5'- uCCCCCCCGccuGAGAAugcucucgcuugGAAAcGGACCGc- -3' miRNA: 3'- -GGGGGGGU---UUUUU------------UUUUcCUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 4594 | 0.66 | 0.999986 |
Target: 5'- uCUCCCCCAcauccggcuccGGAGCCGg- -3' miRNA: 3'- -GGGGGGGUuuuuuuuuu--CCUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 144386 | 0.67 | 0.999978 |
Target: 5'- aCUCUUCCGAAAcgGAAAAGGGAAUCu-- -3' miRNA: 3'- -GGGGGGGUUUU--UUUUUUCCUUGGcac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 125301 | 0.67 | 0.999978 |
Target: 5'- cUCCCCaCCAAucGAGGAA-GAACCuGUGc -3' miRNA: 3'- -GGGGG-GGUUuuUUUUUUcCUUGG-CAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 62179 | 0.67 | 0.999959 |
Target: 5'- uCCCCCaCCAGu------GGGuucAACCGUGa -3' miRNA: 3'- -GGGGG-GGUUuuuuuuuUCC---UUGGCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 10841 | 0.67 | 0.999951 |
Target: 5'- cCCCCCCCAcaaaaaaaaacaaaaAAAAAAAacugucuuauAAGGAGCa-UGg -3' miRNA: 3'- -GGGGGGGU---------------UUUUUUU----------UUCCUUGgcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 6553 | 0.67 | 0.999945 |
Target: 5'- cCCCCCCCGuguuacAAAuucgcguuguuuGAGAuauGGGACgGUGg -3' miRNA: 3'- -GGGGGGGU------UUU------------UUUUuu-CCUUGgCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 4816 | 0.67 | 0.999944 |
Target: 5'- aCCCCCCCuccucccgaugcGGAGGAGCUuUGc -3' miRNA: 3'- -GGGGGGGuuuuuuu-----UUUCCUUGGcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 4939 | 0.67 | 0.999944 |
Target: 5'- aCCCCCCCuccucccgauacGGAGGAACUuUGc -3' miRNA: 3'- -GGGGGGGuuuuuuu-----UUUCCUUGGcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 143491 | 0.67 | 0.999927 |
Target: 5'- --aCCCCAAGAcccGAAAGGGACCcUGc -3' miRNA: 3'- gggGGGGUUUUuu-UUUUCCUUGGcAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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