Results 21 - 40 of 54 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8650 | 5' | -46.1 | NC_002229.2 | + | 125301 | 0.67 | 0.999978 |
Target: 5'- cUCCCCaCCAAucGAGGAA-GAACCuGUGc -3' miRNA: 3'- -GGGGG-GGUUuuUUUUUUcCUUGG-CAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 126284 | 0.71 | 0.996358 |
Target: 5'- gCUCCCCAGGGuGGAAAAGGGGCgGa- -3' miRNA: 3'- gGGGGGGUUUU-UUUUUUCCUUGgCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 127871 | 0.67 | 0.999951 |
Target: 5'- cCCCCCCCAcaaaaaaaaacaaaaAAAAAAAacugucuuauAAGGAGCa-UGg -3' miRNA: 3'- -GGGGGGGU---------------UUUUUUU----------UUCCUUGgcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 128980 | 0.76 | 0.950326 |
Target: 5'- gCCCCCCCAGAAAGAauaaucacugcuucGAAcGGAGCuCGa- -3' miRNA: 3'- -GGGGGGGUUUUUUU--------------UUU-CCUUG-GCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 131797 | 0.66 | 0.999992 |
Target: 5'- -aCCCCCGAG---GGAAGGAAaaaGUGc -3' miRNA: 3'- ggGGGGGUUUuuuUUUUCCUUgg-CAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 132158 | 0.67 | 0.999945 |
Target: 5'- cCCCCCCCGuguuacAAAuucgcguuguuuGAGAuauGGGACgGUGg -3' miRNA: 3'- -GGGGGGGU------UUU------------UUUUuu-CCUUGgCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 133772 | 0.67 | 0.999944 |
Target: 5'- aCCCCCCCuccucccgauacGGAGGAACUuUGc -3' miRNA: 3'- -GGGGGGGuuuuuuu-----UUUCCUUGGcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 133895 | 0.67 | 0.999944 |
Target: 5'- aCCCCCCCuccucccgaugcGGAGGAGCUuUGc -3' miRNA: 3'- -GGGGGGGuuuuuuu-----UUUCCUUGGcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 134117 | 0.66 | 0.999986 |
Target: 5'- uCUCCCCCAcauccggcuccGGAGCCGg- -3' miRNA: 3'- -GGGGGGGUuuuuuuuuu--CCUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 138694 | 0.68 | 0.999791 |
Target: 5'- aCCCCggCCCAcaGGAAGGGgcGGGGCaCGUGc -3' miRNA: 3'- -GGGG--GGGU--UUUUUUUuuCCUUG-GCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 142314 | 0.7 | 0.998991 |
Target: 5'- gCCCUCCCGGGGGAGuaucuGGAGCgGg- -3' miRNA: 3'- -GGGGGGGUUUUUUUuuu--CCUUGgCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 143491 | 0.67 | 0.999927 |
Target: 5'- --aCCCCAAGAcccGAAAGGGACCcUGc -3' miRNA: 3'- gggGGGGUUUUuu-UUUUCCUUGGcAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 144386 | 0.67 | 0.999978 |
Target: 5'- aCUCUUCCGAAAcgGAAAAGGGAAUCu-- -3' miRNA: 3'- -GGGGGGGUUUU--UUUUUUCCUUGGcac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 146819 | 0.7 | 0.998323 |
Target: 5'- cUCCCCCCAGAAAuguccccggauaaucGAacugccGAGGGAACCc-- -3' miRNA: 3'- -GGGGGGGUUUUU---------------UU------UUUCCUUGGcac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 147054 | 0.77 | 0.915038 |
Target: 5'- cUCCCCCCAGAGAAGAAGucGAACUGa- -3' miRNA: 3'- -GGGGGGGUUUUUUUUUUc-CUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 147121 | 0.77 | 0.932313 |
Target: 5'- gCCCCCCGAGuac--GGGGGGCCGg- -3' miRNA: 3'- gGGGGGGUUUuuuuuUUCCUUGGCac -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 147399 | 0.68 | 0.99977 |
Target: 5'- uCCCCCCCGAGcccuccccAGGcuCCGUc -3' miRNA: 3'- -GGGGGGGUUUuuuuuu--UCCuuGGCAc -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 147787 | 0.7 | 0.998503 |
Target: 5'- -gUCCCCGGAuuc-GGGGGAACCGUc -3' miRNA: 3'- ggGGGGGUUUuuuuUUUCCUUGGCAc -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 150422 | 0.66 | 0.999994 |
Target: 5'- ------aAAAAAAAAGAGGAACCGUGc -3' miRNA: 3'- gggggggUUUUUUUUUUCCUUGGCAC- -5' |
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8650 | 5' | -46.1 | NC_002229.2 | + | 150468 | 1.11 | 0.024115 |
Target: 5'- cCCCCCCCAAAAAAAAAAGGAACCGUGc -3' miRNA: 3'- -GGGGGGGUUUUUUUUUUCCUUGGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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