Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8808 | 5' | -60.8 | NC_002484.1 | + | 904 | 0.7 | 0.228063 |
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Target: 5'- gUGGUGGCGCCGgaGUagaccuCGGCCCCCgacucGCCa -3' miRNA: 3'- -ACCGUCGCGGU--UAgc----GUCGGGGG-----UGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 1551 | 0.69 | 0.258032 |
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Target: 5'- cGGCAGCcuGCCAuGUCGCAaucgugaagucgcGCUUCUGCCg -3' miRNA: 3'- aCCGUCG--CGGU-UAGCGU-------------CGGGGGUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 4443 | 0.69 | 0.258676 |
|
Target: 5'- cGaCAGCGCCAggcauaucgGUCGgGGugucaccaCCCCCGCCa -3' miRNA: 3'- aCcGUCGCGGU---------UAGCgUC--------GGGGGUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 14738 | 0.69 | 0.263875 |
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Target: 5'- -cGCAGCGCgGcgguauugaugagGUCGUaguagccGGCCUCCACCu -3' miRNA: 3'- acCGUCGCGgU-------------UAGCG-------UCGGGGGUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 8736 | 0.69 | 0.278608 |
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Target: 5'- cGGCAcugaGUCAG-CGCAGCgCCCAUCg -3' miRNA: 3'- aCCGUcg--CGGUUaGCGUCGgGGGUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 37095 | 0.69 | 0.285519 |
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Target: 5'- aGGCAGCccguaCCAAggGCaaGGCCCUgGCCg -3' miRNA: 3'- aCCGUCGc----GGUUagCG--UCGGGGgUGG- -5' |
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| 8808 | 5' | -60.8 | NC_002484.1 | + | 40417 | 0.66 | 0.432851 |
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Target: 5'- gUGGCGGCGCUgcuuUCGUcgGGCCgCUgACUc -3' miRNA: 3'- -ACCGUCGCGGuu--AGCG--UCGG-GGgUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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