Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8879 | 3' | -56.2 | NC_002484.1 | + | 35428 | 0.71 | 0.409124 |
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Target: 5'- gAUGGCCUaguCGGCGACA--ACGGCCu -3' miRNA: 3'- -UGUCGGGguuGCUGCUGUacUGCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 44491 | 0.71 | 0.412807 |
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Target: 5'- uACGGCCaggaCGugGGCGGCGccgucagcuaccgaGACGGCCc -3' miRNA: 3'- -UGUCGGg---GUugCUGCUGUa-------------CUGCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 55452 | 0.7 | 0.437241 |
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Target: 5'- -gAGCCCauaAACGuCGACAUcaGAUGGCUc -3' miRNA: 3'- ugUCGGGg--UUGCuGCUGUA--CUGCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 9850 | 0.7 | 0.443961 |
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Target: 5'- gGCGGCCCCAcagguaguucuccgGCGAcccCGAUAUGGuCuGCCa -3' miRNA: 3'- -UGUCGGGGU--------------UGCU---GCUGUACU-GcCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 38371 | 0.7 | 0.446858 |
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Target: 5'- aGCuGCaCCCGgcaauACGACGACAUGcGCcGCCg -3' miRNA: 3'- -UGuCG-GGGU-----UGCUGCUGUAC-UGcCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 11426 | 0.69 | 0.506863 |
|
Target: 5'- cCGGCgCUCGGCGGCaucCAUGcCGGCCa -3' miRNA: 3'- uGUCG-GGGUUGCUGcu-GUACuGCCGG- -5' |
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| 8879 | 3' | -56.2 | NC_002484.1 | + | 28420 | 0.66 | 0.699757 |
|
Target: 5'- gGCGGCCgCCGGCauucuCGGCGUGA--GCCg -3' miRNA: 3'- -UGUCGG-GGUUGcu---GCUGUACUgcCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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